Method and apparatus for determination of glycosylated protein

Details Method and apparatus for determination of glycosylated protein Method and apparatus for determination of glycosylated protein

1996-08-20

1998-09-15

Pyon, Harold Y.

Chemistry: analytical and immunological testing

Hemoglobin, myoglobin, or occult blood

Glycosylated hemoglobin

436 15, 436 63, 436 86, 436 66, 436164, 436165, 436169, 436172, 422 56, 422 58, G01N 3372

Patent

active

058077472

ABSTRACT:
A method of determining the glycosylated fraction of a protein in a sample includes the steps of examining the sample to determine the total quantity of protein in the sample (READ 1), removing the glycosylated fraction of the protein, and then reexamining the sample to measure the glycosylated fraction of the protein (READ 2). The glycosylated fraction of the protein may then be calculated by the formula (READ 1-READ 2)/READ .times.100. The glycosylated fraction of the protein is removed by inserting a cartridge containing a binder into the sample, or by urging the sample through a cartridge containing a binder. Both of the cartridge assemblies preferably include a flow through body, a support material disposed in the body, and a binder material attached to the support wherein the binder has an affinity for the glycosylated fraction of the protein.

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