Chemistry: molecular biology and microbiology – Condition responsive control process
Patent
1997-08-04
1999-06-15
Smith, Lynette F.
Chemistry: molecular biology and microbiology
Condition responsive control process
4352521, 4352542, 4352551, 4352568, 4352891, 435822, 435948, C12Q 300, C12N 112, C12N 120, C12N 114
Patent
active
059121130
ABSTRACT:
The present invention is a method for aerobically cultivating yeast or bacteria in a culture medium of fed-batch, continuous or cell-recycling continuous cultures, wherein the carbon source concentration in the culture medium is maintained at a constant low level of under g/l. The carbon source concentration is maintained by measuring the carbon consumption of a culture of the yeast or bacteria in a preliminary experiment. The rate is determined between the time the culture is started and a time when the carbon source is exhausted. A feeding time is then determined wherein the activity of the yeast or bacteria in the presence of the carbon source does not change and a volume of the carbon source to be used in a first feeding (So) is set as So=.nu..times.T. Then, in a main culture, a first feeding of a volume of the carbon source (So) is added for the time (T), and the exhaustion of the carbon source is detected as an increase in pH or an increase in concentration of oxygen dissolved in the culture medium. A second feeding of a volume of the carbon source is then added for the time (T), and the feeding rate is determined based on a period (.tau.) before which the second feeding is added as follows:
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Koyama Yosuke
Miwa Harufumi
Nakamura Takashi
Nakayama Tatsuya
Shimazaki Keishi
Ajinomoto Co. Inc.
Nelson Brett
Smith Lynette F.
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