Method and apparatus for amplifying nucleic acids

Details Method and apparatus for amplifying nucleic acids Method and apparatus for amplifying nucleic acids

2005-03-03

2009-08-25

Kim, Young J (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120, C435S091100

Reexamination Certificate

active

07579172

ABSTRACT:
An apparatus for amplifying nucleic acids includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate, wherein the reactant aqueous solution contacts the fluid to create a plurality of droplets surrounded by the fluid in the reaction vessel and the nucleic acids are amplified in the reactant aqueous solution droplets.

REFERENCES:
patent: 5958694 (1999-09-01), Nikiforov
patent: 2003505059 (2003-02-01), None
patent: WO 93/22053 (1993-11-01), None
patent: 0107259 (2001-02-01), None
patent: WO 01/07159 (2001-02-01), None
European Search Report; App. No. 05004816.40-EP 05 00 4816; Date of Completion: Jul. 4, 2005.
European Search Report for Application No. EP 05 00 4816; Date of completion of search: Oct. 12, 2005.
“Chemical Amplification: Continuous-Flow PCR On A Chip”, Authors: Martin U. Kopp, Andrew J. de Mello, Andreas Manz, Science, vol. 280, pp. 1046-1048, Dated: May 15, 1998.
“A Microfluidic System for Controlling Reaction Networks In Time”, Authors: Helen Song, Joshua D. Tice, and Rustem F. Ismagilov, pp. 767-772, Angew. Chem. Int. Ed. 2003, 42, No. 7.
“Selective Infection of Human CD4+ Cells by Simian Immunodeficiency Virus: Productive Infection Associated with Envelope Glycoprotein-Induced Fusion”, Authors: Scott Koenig, Vanessa M. Hirsch, Robert A. Olmstead, Douglas Powell, Wendy Maury, Arnold Rabson, Anthony S. Fauci, Robert H. Purcell, and Philip R. Johnson, Proc. Natl. Acad. Sci. USA, vol. 86, pp. 2443-2447, Dated: Apr. 1989.
“Single Molecule Amplification in a Continuous Flow Labchip Device”, Authors: Jill Baker, Michelle Strachan, Ken Swartz, Yevgeny Yurkovetsky, Aaron Rulison, Carlton Brooks and Anne Kopf-Sill, 7th International Conference on Miniaturized Chemical and Biochemical Analysis Systems, pp. 1335-1338, Dated: Oct. 5-9, 2003.
“Single-Molecule PCR Using Water-In-Oil Emulsion”, Authors: Michihiko Nakano, Jun Komatsu, Shun-ichi Matsuura, Kazunori Takashima, Shinji Katsura, Akira Mizuno, Journol of Biotechnology 102 (2003), pp. 117-124.
“Single-Molecule DNA Amplification and Analysis in an Integrated Microfluidic Device”, Authors: E.T. Lagally, I. Medintz, and R.A. Mathies, Analtical Chemistry, vol. 73, No. 3, Dated: Feb. 1, 2001, pp. 565-570.
Japanese Office Action for Application No. 2005-071485 dated Nov. 27, 2007, citing the references in the instant IDS.
Chiou et al; “A Closed-Cycle Capillary Polymerase Chain Reaction Machine” Analytical Chemistry, vol. 73, No. 9, 2001, pp. 2018-2021.
Handbook of Lectures of the 49th Joint Lecture Meeting of the Applied Physics, vol. 3, 2002, p. 1298.

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