Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2011-05-17
2011-05-17
Kim, Young J (Department: 1637)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S006120, C435S091100
Reexamination Certificate
active
07943348
ABSTRACT:
A method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.
REFERENCES:
patent: 5939261 (1999-08-01), Loewy et al.
patent: 5958694 (1999-09-01), Nikiforov
patent: 7595195 (2009-09-01), Lee et al.
patent: 2002/0068357 (2002-06-01), Mathies et al.
patent: 2003505059 (2003-02-01), None
patent: 93/22053 (1993-11-01), None
patent: 01/07159 (2001-02-01), None
Japanese Office Action for application No. 2005-071485 dated Nov. 27, 2007.
European Search Report: App. No. 05004816.4-EP 05 00 4816; Date of Completion: Jul. 4, 2005.
European Search Report for Application No. EP 05 00 4816; Date of completion of search: Oct. 12, 2005.
Chiou, et al., “A Closed-Cycle Capillary Polymerase Chain Reaction Machine” Analytical Chemistry, vol. 73, No. 9, 2001, pp. 2018-2021.
Handbook of Lectures of the 49th Joint Lecture Meeting of the Applied Physics, vol. 3, 2002, p. 1298.
Kopp, et al., “Chemical Amplification: Continuous-Flow PCR on a Chip”, Science, vol. 280, pp. 1046-1048, Dated: May 15, 1998.
Song, et al., “A Microfluidic System for Controlling Reaction Networks in Time”, Angew. Chem. Int. Ed. 2003, 42, No. 7, pp. 768-772.
Koenig, et al., “Selective Infection of Human CD4+Cells by Simian Immunodeficiency Virus: Protective Infection Associated With Envelope Glycoprotein-Induced Fusion”, Proc. Natl. Acad. Sci. USA, vol. 86, pp. 2443-2447, Dated: Apr. 1989.
Baker, et al., “Single Molecule Amplification in a Continuous Flow Labchip Device”, 7th International Conference on Miniaturized Chemical and Biochemical Analysis Systems, pp. 1335-1338, Dated: Oct. 5-9, 2003.
Nakano, et al., “Single-Molecule PCR using Water-in-Oil Emulsion”, Journal of Biotechnology 102 (2003) 117-124.
Lagally, et al., “Single-Molecule DNA Amplification and Analysis in an Integrated Microfluidic Device”, Analytical Chemistry, vol. 73, No. 3, Dated: Feb. 1, 2001, pp. 565-570.
Cho Yoon-kyoung
Kim Joon-Ho
Lim Geun-bae
Min Jun-hong
Namkoong Kak
Cantor & Colburn LLP
Kim Young J
Samsung Electronics Co,. Ltd.
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