Method and algorithm for quantifying polynucleotides

Details Method and algorithm for quantifying polynucleotides Method and algorithm for quantifying polynucleotides

2006-06-22

2010-06-15

Moran, Marjorie (Department: 1631)

Data processing: measuring, calibrating, or testing

Measurement system in a specific environment

Biological or biochemical

C435S006120

Reexamination Certificate

active

07739054

ABSTRACT:
Machine executable method of analyzing growth curve data to identify the transition from a baseline phase into a growth phase. Applications of the method include analysis of results from time-dependent monitoring of amplicon synthesis in a nucleic acid amplification reaction to quantify a starting amount of a nucleic acid template in a test sample. The method advantageously simplifies the quantitation by circumventing the need to establish thresholds used for calculating initiation of the growth phase, to calculate derivatives, or to perform linear regression analysis.

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