Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1995-06-06
1997-12-23
Walsh, Stephen
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
435 71, 435 72, 435 691, 43525233, 435325, 4352542, 530300, 530350, 536 231, 536 235, C12N 121
Patent
active
057006822
ABSTRACT:
A method is described for screening retinoid X receptor agonists or antagonists using a retinoid X receptor expressed by a yeast expression system to screen a compound having a retinoid X receptor agonist or antagonist activity. Another screen is described for detecting a compound having retinoid X receptor agonist or antagonist activity by: (1) providing a yeast strain which expresses the retinoic acid receptor and activates a reporter plasmid containing apolipoprotein AI gene site A or a mutated variant thereof; (2) incubating the compound in suitable media and a colorless chromogenic substrate; and (3) examining the media for development of color. Mutated variants of apolipoprotein AI gene site A which respond selectively to the retinoid X receptor or respond to receptors other than RXR.alpha. are also described.
REFERENCES:
Rottman et al., Molecular and Cellular Biology, 11:3814-3820 (1991).
Windom et al., Molecular and Cellular Biology, 12:3380-3389 (1992).
Karathansis: "Apolipoprotein Al gene regulation by members of the steroid/thyroid hormone . . . ", pp. 21-32 of High density lipoproteins and atherosclerosis III, (Miller et al. eds. 1992).
Ladias et al. (1991) Science 251:561-565.
Mak et al. (1989) J. Biol. Chem. 214:21613-21618.
McDonnell et al. (1991) J. Ster. Bioch. Mol. Biol. 39:291-297.
Yu et al. (1991) Cell 67:1251-1266.
Karathanasis Sotirios K.
Mak Paul
American Cyanamid Company
Barnhard Elizabeth M.
Sorensen Kenneth A.
Walsh Stephen
LandOfFree
Mechanism based screen for retinoid X receptor agonists and anta does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Mechanism based screen for retinoid X receptor agonists and anta, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Mechanism based screen for retinoid X receptor agonists and anta will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1802012