Hepadnavirus polymerase gene product having RNA-dependent DNA pr

Details Hepadnavirus polymerase gene product having RNA-dependent DNA pr Hepadnavirus polymerase gene product having RNA-dependent DNA pr

1993-08-02

1994-11-01

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 15, 435 691, 435 9151, 935 14, 935 17, 25218311, 536 232, 536 2372, C12Q 148, C12Q 168, C08L 500, C12N 912

Patent

active

053607147

ABSTRACT:
An enzymatically active composition of matter, comprising an extracellular combination of a substantially virus-free hepadnavirus polymerase gene product and an RNA template for hepadnavirus minus strand DNA synthesis, is provided. The composition possesses a DNA priming activity and a reverse transcriptase activity. The hepadnavirus polymerase gene product is produced by in vitro expression of a hepadnavirus pol gene.
The enzymatically active composition of matter is used in rapid in vitro assays to screen potential anti-hepadnaviral agents. Assays for various functions of the hepadnavirus DNA polymerase are provided, including: (1) binding of the polymerase gene product to hepadnavirus pre-genomic RNA; (2) DNA priming activity of the polymerase gene product; and (3) reverse transcriptase activity of the polymerase gene product.

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