Means for determining percentage of glycohemoglobin in whole blo

Details Means for determining percentage of glycohemoglobin in whole blo Means for determining percentage of glycohemoglobin in whole blo

1981-03-26

1987-03-10

Turk, Arnold

Chemistry: analytical and immunological testing

Hemoglobin, myoglobin, or occult blood

Glycosylated hemoglobin

436 66, G01N 3372

Patent

active

046491228

ABSTRACT:
A method is provided for determining the percentage of glycohemoglobin in patient whole blood. The method comprises subjecting total hemoglobin comprising non-carbohydrate fixed hemoglobin and glycohemoglobin of samples of (1) patient whole blood lysate P and (2) whole blood lysates K.sub.1 and K.sub.2 of known glycohemoglobin percentages %.sub.1 and %.sub.2, to batchwise selective adsorption on an adsorbent which is specific either for the hemoglobin or for the glycohemoglobin, thereby adsorbing each sample under the same conditions and leaving in solution the corresponding non-adsorbed total hemoglobin fractions P.sub.f, K.sub.1f and K.sub.2f. The ratios R.sub.p, R.sub.k1 and R.sub.k2 of the optical density of the total hemoglobin fractions P.sub.f, K.sub.1f and K.sub.2f to the respective optical density of total hemoglobin aliquot samples of lysates P, K.sub.1 and K.sub.2 are measured, and the percentage of glycohemoglobin relative to total hemoglobin for each patient blood sample is calculated.

REFERENCES:
patent: 3871832 (1975-03-01), Leblanc
patent: 3996162 (1976-12-01), McCall
patent: 4161104 (1979-08-01), Wagner et al.
patent: 4238196 (1980-12-01), Acuff et al.
patent: 4268270 (1981-05-01), Gabbay et al.
patent: 4269605 (1981-05-01), Dean et al.
Henry, "Clinical Chemistry--Principles and Techniques", Harper and Row, 1964, pp. 732-734.

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