Mammalian/yeast hybrid G protein-coupled receptors

Details Mammalian/yeast hybrid G protein-coupled receptors Mammalian/yeast hybrid G protein-coupled receptors

1994-02-07

1996-11-19

Ulm, John

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Fungi

435 647, 4352523, 4353201, 536 234, C12N 119, C12N 1562

Patent

active

055762107

ABSTRACT:
Methods are disclosed for producing hybrid G protein-coupled receptors. DNA sequences encoding hybrid G protein-coupled receptors are provided, wherein the receptors comprise mammalian G protein-coupled receptors having at least one domain other than the ligand-binding domain replaced with a corresponding domain of a yeast G protein-coupled receptor. DNA constructs comprising the following operatively linked elements: a transcriptional promoter, a DNA sequence encoding a hybrid G protein-coupled receptor, wherein the receptor comprises a mammalian G protein-coupled receptor having at least one domain other than the ligand-binding domain replaced with a corresponding domain of a yeast G protein-coupled receptor, and a transcriptional terminator. Host cells transformed with the DNA constructs and methods utilizing the transformed cells are also provided.

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