Macroporous and microporous inorganic carrier for immobilization

Details Macroporous and microporous inorganic carrier for immobilization Macroporous and microporous inorganic carrier for immobilization

1989-12-29

1992-03-17

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

210601, 210606, 264 43, 435176, 43524023, 43524024, 435288, C12P 100, C02N 1114, C02N 500, C02F 300

Patent

active

050968149

ABSTRACT:
For the immobilization of micro-organisms and animal cells, in particular for anaerobic processes, such as the purification of waste water or for the biotechnological production of nutrition-essential or pharmacological substances, porous, sintered bodies are employed (inorganic carrier bodies). In particular, sintered glass in the form of Raschig rings with a double-pore structure, are employed. They have porosity-determining through-going macropores that permit a free exchange of fluid and gas from the interior of the carrier to the surroundings, and open micropores within the macropore walls, the diameter of the micropores being of the same order of magnitude as the size of the micro-organisms or cells. These carrier bodies typically have an open pore volume of 35% to 85%, 20% to 80% being accounted for by the macropores having a diameter of 20 to 500 .mu.m, and 5%-50% by micropores having a diameter of 1-10 .mu.m. These bodies are obtained by sintering a powder mixture comprising fine-grain material and a coarse-grain substance melting at a higher-than-sintering temperature and separable from the sintered product by allowing the latter to cool and separating (dissolving) out the soluble component.

REFERENCES:
patent: 3992329 (1976-11-01), Eaton et al.
patent: 4153510 (1979-05-01), Messing et al.
patent: 4321141 (1982-03-01), Messing
patent: 4588540 (1986-05-01), Kiefer et al.
Durand et al., Process Biochemistry, Sep., 1978, pp. 14-23.
Huysman et al., "Factors Affecting the Colonization of Non Porous and Porous Packing Materials in Model Upflow Methane Reactors", Biotechnology Letters, vol. 5, No. 9, pp. 643-648 (1983).

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