Lyophilization of bacteria

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Preserving or maintaining micro-organism

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426 36, 426 43, 426 61, 426385, 435261, 435885, C12K 108, C12K 300, A23C 1902

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active

042051320

ABSTRACT:
Storage stable, lyophilized, acid producing bacteria, such as lactic acid bacteria, are prepared by lyophilizing the bacteria in the presence of at least 5% by weight of the bacteria of a basic organic or inorganic buffering agent, preferably an alkali metal salt of glycerophosphate, to provide lyophilized bacteria containing less than 5% by weight water, and sealing the lyophilized bacteria in a container while in a vacuum or surrounded by a gaseous atmosphere substantially free of oxygen, preferably argon which is essentially free of oxygen.

REFERENCES:
patent: 3592740 (1971-07-01), Christensen
patent: 3897307 (1975-07-01), Porubcan et al.
patent: 3968256 (1976-07-01), Sing
patent: 3975545 (1976-08-01), Vedamuthu
Speckman et al., Lyophilized Lactic Acid Starter Culture Concentrates, J. Da. Sci., vol. 57, No. 2, 1974 (pp. 165-173).
Baumann et al., Freezing of Lactic Cultures, J. Da. Sci., vol. 49, 1966 (pp. 259-264).
Cowman et al., Activity of Lactic Streptococci Following Ultra-Low-Temperature Storage, J. Da. Sci., vol. 46, 1963 (p. 609).
Douglas et al., Medium for the Propagation and Assay of Lactic and Other Phages, Laboratory Practice, vol. 23, 1974 (pp. 3-5).
Douglas, J., A Critical Review of the Use of Glycerophosphates in Microbiological Media, Laboratory Practice, vol. 20, 1971 (pp. 414-417).
Terzaghi et al., Improved Medium for Lactic Streptococci and Their Bacteriophages, Applied Microbiology, vol. 29, No. 6, 1975 (pp. 807-813).

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