Low-molecular weight peptide mixture and method of producing sam

Details Low-molecular weight peptide mixture and method of producing sam Low-molecular weight peptide mixture and method of producing sam

1985-11-01

1990-07-10

Teskin, Robin L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 68, 435212, 435219, 435272, 426651, 426656, 426 46, 426 49, 426 52, 426 18, C12P 2100, C12P 2106, C12N 948, C07K 312

Patent

active

049406620

ABSTRACT:
A method of producing a low-molecular weight peptide mixture, comprising the steps of dissolving a first protease in a buffer solution adjusted to an optimum pH for the protease ranging from pH 3 to 10, adding at least one first protein in a buffer solution having a pH of from 3 to 10 and a concentration of 10 to 60% by weight of the protein material and thoroughly mixing the solution, adding a solution of an ester of at least one amino acid pre-formed by esterification of the amino acid with an alcohol, the pH of said buffer solution being optimum for the incorporation of said amino acid in said starting protein in the presence of said first protease in a plastein reaction, reacting said ester of at least one amino acid with said starting protein in a plastein reaction in the mixed solution whereby said at least one amino acid is covalently incorporated into said starting protein to produce a plastein reaction solution containing a modified protein, hydrolyzing said modified protein using at least one second protease having a different specificity from said at least one first protease to produce a low-molecular weight peptide mixture having an amino acid content which has different proportions of amino acids than does said starting protein, and separating from the solution said low-molecular weight peptide mixture which comprises a major proportion of dipeptides and tripeptides and containing not more than 15% by weight of free amino acids and not more than 20% by weight of a high-molecular weight fraction of compounds having a molecular weight of not lower than 700 and removable by gel filtration. The said low-molecular weight peptide mixture is readily absorbable and a useful nutrient.

REFERENCES:
patent: 3803327 (1974-04-01), Fujimaki et al.
patent: 3904519 (1975-09-01), McKinney et al.
patent: 4016147 (1977-04-01), Fujimaki et al.
patent: 4087388 (1978-05-01), Jensen et al.
patent: 4145455 (1979-03-01), Fujimaki et al.
patent: 4452888 (1984-06-01), Yamazaki et al.
Yamashita et al., J. Agric. Food Chem., vol. 27(1), 1979, "A One-Step Process for Incorporation of L-Methionine into Soy Protein by Treatment with Papain".
Hanson, "Peptides 1972," (1973), pp. 355-362.
Chem. Abstracts, vol. 92, (1980), 126939f.
Chem. Abstracts, vol. 90, (1979), 70795v.
Chem. Abstracts, vol. 91, (1979), 54886r.

Affiliated with

Also associated with

Rating

Low-molecular weight peptide mixture and method of producing sam does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Low-molecular weight peptide mixture and method of producing sam, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Low-molecular weight peptide mixture and method of producing sam will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1720679