Chemistry: analytical and immunological testing – Hemoglobin – myoglobin – or occult blood – Glycosylated hemoglobin
Patent
1990-05-14
1992-09-29
Lacey, David L.
Chemistry: analytical and immunological testing
Hemoglobin, myoglobin, or occult blood
Glycosylated hemoglobin
436 66, 436 17, G01N 3372, G01N 3350
Patent
active
051513692
ABSTRACT:
Use of a lithium salt, particularly lithium thiocyanate, as an agent for lysing red blood cells and denaturing hemoglobin in the performance of an immunoassay to detect the relative amount of a hemoglobin derivative in a blood sample. The method is particularly useful in the determination of hemoglobin A1c. The method rapidly releases hemoglobin from red blood cells by lysis and exposes the epitope that is characteristic of the A1c form. Low concentrations of the lithium salt provide rapid release and denaturation of hemoglobin without the need for dilution prior to the performance of the immunoassay.
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Lewis Lynette A.
Yip M. Teresa
Daley Thomas E.
Lacey David L.
Miles Inc.
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