Cleaning compositions for solid surfaces – auxiliary compositions – Cleaning compositions or processes of preparing – Enzyme component of specific activity or source
Reexamination Certificate
2001-01-30
2003-04-01
Gupta, Yogendra N. (Department: 1751)
Cleaning compositions for solid surfaces, auxiliary compositions
Cleaning compositions or processes of preparing
Enzyme component of specific activity or source
C510S276000, C510S300000, C510S320000, C510S374000, C510S305000, C435S209000
Reexamination Certificate
active
06541438
ABSTRACT:
FIELD OF THE INVENTION
The present invention relates to laundry detergent and/or fabric care compositions comprising a modified enzyme which comprises a catalytically active amino acid sequence of a cellulolytic enzyme linked to an amino acid sequence comprising a Cellulose Binding Domain (CBD) having a relative binding constant (Kr-a) for binding to amorphous cellulose higher than 2.4 l/gcellulose, preferably higher than 3.5 /gcellulose, more preferably higher than 4 l/gcellulose.
BACKGROUND OF THE INVENTION
Detergent compositions include nowadays a complex combination of active ingredients and in particular, detergent enzymes such as cellulases.
The activity of cellulase is one in which cellulosic fibres or substrates are attacked by the cellulase and is depending on the particular function of the cellulase, which can be endo- or exo- cellulase and on the respective hemicellulases. The cellulose structures are depolymerized or cleaved into smaller and thereby more soluble or dispersible fractions. This activity in particular on fabrics provides cleaning, rejuvenation, softening and generally improved handfeel characteristics to the fabric structure. This has been previously speculated to be the result of the cleavage of fibrils from the surface of fibres such that the main strand of the fibre becomes smoother, less available for incrustation, less likely to entangle with other fibers and optically less diffuse in its light reflecting and emission.
Improvements in the activity of particular cellulase compositions have been made over time in order to allow lower usage or shorter exposure times for similar benefits. In the detergent field, cellulases performing in a typical detergent wash environment are available with an activity at which the desired cellulase performance is reached prior to the end of a wash-cycle. However, since the cellulase continues to react, even after having provided the desired performance, cleavage of the cellulose will continue. Therefore, there is a potential risk of tensile strength loss.
It should be noted however, that tensile strength loss of fabric is also an unavoidable result of mechanical action due to use/wearing and may further result from damage by a bleaching component in the laundry process, especially if the fabric is contaminated with metal compounds.
The object of the present invention is to provide laundry detergent and/or fabric care compositions which achieve excellent fabric care, including anti-wrinkle, anti-bobbling and anti-shrinkage properties to fabrics, as well as provide static control, fabric softness, colour appearance and fabric anti-wear properties and benefits, and which achieve excellent fabric cleaning while preventing tensile strength loss.
The above objective has been met by formulating laundry detergent and/or fabric care compositions comprising a modified enzyme which comprises a catalytically active amino acid sequence of a cellulolytic enzyme linked to an amino acid sequence comprising a Cellulose Binding Domain (CBD) having a relative binding constant (Kr-a) for binding to amorphous cellulose higher than 2.4 l/gcellulose, preferably higher than 3.5 l/gcellulose, more preferably higher than 4 l/gcellulose.
WO91/10732 discloses an enzyme which exhibits cellulase activity, producible by a strain of Bacillus spp., NICMB 40250, especially an 75, 56 or 45 kD Molecular weight endoglucanase or characterised by a high specific activity for e.g. detergency application, providing softening, soil removal and colour clarification. Novel derivatives of cellulase enzymes combining a core region derived from said endoglucanase with a CBD derived from another cellulase enzyme or a combining a core region derived from another cellulase enzyme with a CBD derived from said endoglucanase, are therein contemplated to construct cellulase enzymes with improved binding properties.
WO94/07998 describes cellulase variants of a cellulase classified in family 45, comprising a CBD, a Catalytically Active Domain (CAD) and a region linking the CBD to the CAD, wherein one or more amino acid residues have been added, deleted or substituted and/or another CBD is added at the opposite end of the CAD. These enzymes have improved properties such as alkaline activity, harshness reduction, compatibility with detergent ingredients, particulate soil removal, colour clarification, defuzzing, depilling, harshness reduction and sensitivity to anionic surfactants and peroxidase bleaching systems and are useful in detergent compositions.
WO95/16782 relates to the cloning and high level expression of novel truncated cellulase proteins or derivatives thereof in
Trichoderma longibrachiatum
. Combinations of different core regions with several CBDs are described in the fibre/fabric context.
A cellulolytic enzyme preparation comprising a cellulase with reduced mobility, e.g. by increasing the molecular weight or apparent size of the cellulase protein molecule or by insolubilising or immobilising the cellulase, has been disclosed in WO97/01629. The mobility of the cellulase component may be reduced by adsorption to an insoluble or soluble carrier e.g. via the existing or newly introduced CBD.
However, none of these documents disclose a modified enzyme which comprises a catalytically active amino acid sequence of a cellulolytic enzyme linked to an amino acid sequence comprising a Cellulose Binding Domain having a Kr-a of a definite value, thereby defining a cellulase especially adapted to selectively bind and hydrolyse amorphous cellulose of cotton containing fabrics for a laundry and/or fabric care application.
SUMMARY OF THE INVENTION
The present invention relates a modified cellulolytic enzyme demonstrating selective binding and hydrolysis of amorphous cellulose in cotton containing fabrics. The present invention further relates to a laundry detergent and/or fabric care composition comprising said modified enzyme. Said compositions achieve excellent fabric care and fabric cleaning while preventing tensile strength loss. This modified enzyme comprises a catalytically active amino acid sequence of a cellulolytic enzyme linked to an amino acid sequence comprising a Cellulose Binding Domain (CBD) having a relative binding constant (Kr-a) for binding to amorphous cellulose higher than 2.4 l/gcellulose, preferably higher than 3.5 l/gcellulose, more preferably higher than 4 l/gcellulose.
In a further embodiment of the present invention, the present invention relates to a modified enzyme which comprises a catalytically active amino acid sequence of a cellulolytic enzyme linked to an amino acid sequence comprising a Cellulose Binding Domain further characterised by having a relative binding constant for bacterial microcrystalline cellulose (Kr-c) lower than 1 l/gcellulose, preferably lower than 0.5 l/gcellulose.
More preferably, the modified enzyme of the present invention, comprises a catalytically active amino acid sequence of a cellulolytic enzyme linked to an amino acid sequence comprising a Cellulose Binding Domain further characterised by having a relative binding constant (Kr-a) for binding to amorphous cellulose to a relative binding constant for bacterial microcrystalline cellulose (Kr-c) ratio above 3.
The present invention further relates to laundry detergent and/or fabric care compositions comprising said modified enzyme and which preferably further comprise a detergent ingredient selected from cationic surfactants, dye transfer inhibiting polymer, builders,—in particular zeolite A and sodium tripolyphosphate—and/or clays .
DETAILED DESCRIPTION OF THE INVENTION
The present invention relates to a modified enzyme which comprises a catalytically active amino acid sequence of a cellulolytic enzyme linked to an amino acid sequence comprising a cellulose binding domain having a relative binding constant for binding to amorphous cellulose higher than 2.4 l/gcellulose, preferably higher than 3.5 l/gcellulose, more preferably higher than 4 l/gcellulose.
Cellulolytic Enzyme
A modified enzyme for use in accordance with the present invention comprises a catalyticall
Baeck Andre Cesar
Bettiol Jean-Luc Philippe
Boyer Stanton Lane
Busch Alfred
Smets Johan
Cook C. Brant
Elhilo Eisa
Gupta Yogendra N.
Miller Steven W.
The Procter & Gamble & Company
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