Laundry detergent and/or fabric care compositions comprising...

Cleaning compositions for solid surfaces – auxiliary compositions – Cleaning compositions or processes of preparing – Enzyme component of specific activity or source

Reexamination Certificate

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C510S392000, C510S276000, C510S305000, C510S320000, C510S374000, C435S174000

Reexamination Certificate

active

06468955

ABSTRACT:

FIELD OF THE INVENTION
The present invention relates to laundry detergent and/or fabric care compositions comprising a modified enzyme which comprises a catalytically active amino acid sequence of an enzyme, linked via a non-amino acid linking region to an amino acid sequence comprising a Cellulose Binding Domain (CBD).
BACKGROUND OF THE INVENTION
Modem laundry detergent and/or fabric care compositions contain various detergent ingredients having one or more purposes in obtaining fabrics which are not only clean but also have retained appearance and integrity. Therefore, detergent components such as perfumes, soil release agents, fabric brightening agents, fabric softeners, chelants, bleaching agents and catalysts, dye fixatives and enzymes, have been incorporated in laundry detergent and/or fabric care compositions. One of such specific example is the use of enzymes, especially proteases, lipases, amylases and/or cellulases.
Proteases are commonly used enzymes in cleaning applications. Proteases are known for their ability to hydrolyse other proteins. This ability has been taken advantage of through the incorporation of naturally occurring or engineered protease enzymes in laundry detergent compositions.
The inclusion of lipolytic enzymes in detergent compositions for improved cleaning performance is known, e.g. enhancement of removal of triglycerides containing soils and stains from the fabrics.
Amylase enzymes have long been recognised in detergent compositions to provide the removal of starchy food residues or starchy films from dishware or hard surfaces or to provide cleaning performance on starchy soils as well as other soils typically encountered in laundry applications.
The activity of cellulase is one in which cellulosic fibres or substrates are attacked by the cellulase and is depending on the particular function of the cellulase, which can be endo- or exo-cellulase, and on the respective hemicellulases. The cellulose structures are depolymerized or cleaved into smaller and thereby more soluble or dispersible fractions. This activity in particular on fabrics provides a cleaning, rejuvenation, softening and generally improved handfeel characteristics to the fabric structure.
However, it has been difficult to incorporate enzymes into modern detergents in an effective manner. In that regards, those skilled in the art have sought to use minimal amounts of enzyme to their fullest effectiveness by ensuring that most, if not all, of the enzyme comprised in the detergent composition deposits on the fabric. For example, the optimum cellulase would have a binding domain especially suitable for cellulose. In this way, most of the cellulase enzyme included in the detergent composition deposits or otherwise binds to the fabric during the laundering cycle to achieve its desired results.
Similarly, it would be desirable to have laundry detergent and/or fabric care compositions in which its enzymatic components are also modified to ensure deposition onto the fabrics for improved or new performances.
Accordingly, there remains a need for laundry detergent and/or fabric care enzymes which have improved deposition, i.e. closer and/or more lasting contact, on fabrics to be laundered for improved performance during typical washing/fabric care cycles. There also remains a need for such enzymes which are suitable for use in modern laundry detergent and/or fabric care compositions to be formulated in an effective manner.
The above objectives have been met by formulating laundry detergent and/or fabric care compositions comprising an enzyme which has been modified so as to have increased affinity (relative to unmodified enzyme) for binding to a cellulosic fabric or textile. Said modified enzyme comprises a catalytically active amino acid sequence of an enzyme, linked via a non-amino acid linking region to an amino acid sequence comprising a Cellulose Binding Domain.
Enzymes linked to Cellulose Binding Domains are described in the art WO91/10732 novel derivatives of cellulase enzymes combining a core region derived from an endoglucanase producible by a strain of Bacillus spp., NICMB 40250 with a CBD derived from another cellulase enzyme or combining a core region derived from another cellulase enzyme with a CBD derived from said endoglucanase, for improved binding properties. WO94/07998 describes cellulase variants of a cellulase classified in family 45, comprising a CBD, a Catalytically Active Domain (CAD) and a region linking the CBD to the CAD, wherein one or more amino acid residues have been added, deleted or substituted and/or another CBD is added at the opposite end of the CAD. WO95/16782 relates to the cloning and high level expression of novel truncated cellulase proteins or derivatives thereof in
Trichoderma longibrachiatum
comprising different core regions with several CBDs. WO97/01629 describes cellulolytic enzyme preparation wherein the mobility of the cellulase component may be reduced by adsorption to an insoluble or soluble carrier e.g. via the existing or newly introduced CBD. WO97/28243 describes a process for removal or bleaching or soiling or stains from cellulosic fabrics wherein the fabric is contacted in aqueous medium with a modified enzyme which comprises a catalytically active amino acid sequence of a non-cellulolytic enzyme selected from amylases, proteases, lipases, pectinases and oxidoreductases, linked to an amino acid sequence comprising a cellulose binding domain and a detergent composition comprising such modified enzyme and a surfactant.
However, none of these documents disclose a laundry detergent and/or fabric care composition comprising a modified enzyme which comprises a catalytically active amino acid sequence of an enzyme, linked via a non-amino acid linking region to an amino acid sequence comprising a Cellulose Binding Domain, thereby providing increased or enhanced performance of the laundry detergent and/or fabric care composition.
SUMMARY OF THE INVENTION
The present invention relates to a modified enzyme which comprises a catalytically active amino acid sequence of an enzyme, linked via a non-amino acid linking region to an amino acid sequence comprising a Cellulose Binding Domain. The present invention further relates to laundry detergent and/or fabric care compositions comprising such modified enzyme and which provides increased or enhanced performance of the enzymatic component. In a further embodiment, the present invention relates to the use of such modified enzymes for improved cleaning, improved fabric care and improved sanitisation performance.
DETAILED DESCRIPTION OF THE INVENTION
The Enzymes
The present invention relates to a modified enzyme which comprises a catalytically active amino acid sequence of an enzyme, linked via a non-amino acid linking region to an amino acid sequence comprising a Cellulose Binding Domain. This enzyme modification results in a higher effective concentration of the enzyme at its substrate location and therefore, increased or enhanced enzymatic benefits.
Without wishing to be bound by theory, It has been surprisingly found that said modified enzyme more readily attaches, affixes or otherwise comes into closer and/or more lasting contact with the fabric, thereby resulting in increased or enhanced performance of the enzyme. In particular, the laundry detergent and/or fabric care compositions of the present invention when comprising enzymes so modified, provide improved enzymatic benefits, i.e. improved enzymatic stain removal, enhanced enzymatic fabric care and/or improved enzymatic sanitisation benefits. Said enhanced enzymatic benefits are achieved by means of a process wherein the fabric is contacted with an enzyme which has been modified so as to have increased affinity (relative to unmodified enzyme) for binding to a cellulosic fabric or textile.
Without wishing to be bound by theory, it is believed that the linking of the enzyme to the CBD via non-amino acid linking region results in improved stability of the enzyme hybrid. Indeed, this non-amino acid linking region will not be cleaved by proteolytic de

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