Kit for synthesizing polynucleotides

Details Kit for synthesizing polynucleotides Kit for synthesizing polynucleotides

2008-04-15

2010-12-14

Mummert, Stephanie K (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120

Reexamination Certificate

active

07851186

ABSTRACT:
The present invention realized isothermal and rapid polynucleotide synthesis by using as templates polynucleotides having a structure capable of forming loops, and combining a plurality of primers capable of providing a starting point for complementary strand synthesis to such loops. If the LAMP method is applied, all reactions can be carried out isothermally and rapidly since the template polynucleotides themselves can also be synthesized by an isothermal reaction.

REFERENCES:
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Notomi et al. (Nucleic Acids Research, 2000, vol. 28, No. 12, e63, p. i-vii, IDS reference).
Whitcombe et al. (Nature Biotechnology, 1999, vol. 17, p. 804-807).
Notomi et al., “Loop-Mediated Isothermal Amplification of DNA,” Nucleic Acids Research, 2000, vol. 28, No. 12, p. i-vii.
European Search Report for related European application 08019286.7 (Jan. 29, 2009).

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