Isolation and purification method of biomolecules using...

Details Isolation and purification method of biomolecules using... Isolation and purification method of biomolecules using...

2005-07-27

2009-08-25

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S025400

Reexamination Certificate

active

07579151

ABSTRACT:
Provided is a method of isolating and purifying biomolecules using a hydrogel, the method including: bring a sample containing charged biomolecules into contact with a hydrogel to bind the biomolecules to the hydrogel; washing the hydrogel bound with the biomolecules; and eluting the bound biomolecules using an elution solvent. According to the method, the use of a hydrogel with a large surface area reduces the isolation time of biomolecules to 5 min or less, an external device such as an electromagnet is not required, and small-sized systems or LOC can be easily implemented due to applicability to microsystems through a polymer patterning technique.

REFERENCES:
patent: 5705628 (1998-01-01), Hawkins
patent: 5804684 (1998-09-01), Su
patent: 6258996 (2001-07-01), Goldman
patent: 2003/0008320 (2003-01-01), Baker
patent: 2003/0218130 (2003-11-01), Boschetti et al.
patent: 2004/0157244 (2004-08-01), Budahazi et al.
patent: 2006/0121116 (2006-06-01), Mori et al.
patent: 2001-0101994 (2001-11-01), None
Elaissari et al. Hydrophilic magnetic latex for nucleic acid extraction, purification and concentration. Journal of Magnetism and Magnetic Materials (2001) 225: 127-133.
Dissing et al. Polyelectrolyte complexes as vehicles for affinity precipitation of proteins. Journal of Biotechnology (1996) 52: 1-10.
Nakamae et al. Synthesis and characterization of stimuli-sensitive hydrogels having a different length of ethylene glycol chains carrying phosphate groups: loading and release of lysozyme. Journal of Biomaterials Science Polymer Edition (2004) 15(11): 1435-1446.
Jensen et al. Loading into and electro-stimulated release of peptides and proteins from chondroitin 4-sulphate hydrogels. European Journal of Pharmaceutical Sciences (2002) 15: 139-148.
Olsen et al. Immobilization of DNA hydrogel plugs in microfluidic channels. Analytical Chemistry (2002) 74(6): 1436-1441.
Tarcha et al. Absorption-enhanced solid-phase immunoassay method via water-swellable poly(acrylamide) microparticles. Journal of Immunological Methods (1989) 125: 243-249.
Tabata et al. In vitro sorption and desorption of basic fibroblast growth factor biodegradable hydrogels. Biomaterials (1998) 19: 1781-1789.
Kondo et al. Preparation of thermo-sensitive magnetic hydrogel microspheres and application to enzyme immobilization. Journal of Fermentation and Bioengineering (1997) 84(4): 337-341.
Sato et al. Hydrogel-microsphere-enhanced surface plasmon resonance for the detection of a K-ras point mutation employing peptide nucleic acid. Journal of Biomaterials Science Polymer Edition (2003) 14(8): 803-820.
An Office Action of Korean Intellectual Property Office, regarding corresponding patent applicaton No. 10-2004-0104025.
All the references cited in the Search Report are listed above.
“Rapid, Universal Method to Isolate PCR-Ready DNA Using Magnetic Beads”; Authors: K. Rudi, et al.; BioTechniques vol. 22, No. 3, pp. 506-511 (1997).
“Rapid Isolation of PCR-Ready DNA From Blood, Bone Marrow and Cultured Cells, Based on Paramagnetic Beads”; Authors: Arne Deggerdal and Frank Larsen.

Affiliated with

Also associated with

Rating

Isolation and purification method of biomolecules using... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Isolation and purification method of biomolecules using..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Isolation and purification method of biomolecules using... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4100833