Isolated oxide synthase and divinyl ether synthase and uses...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Plant proteins – e.g. – derived from legumes – algae or...

Reexamination Certificate

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Details

C530S350000, C435S419000, C435S069100

Reexamination Certificate

active

07321025

ABSTRACT:
The present invention relates to enzymes from the cytochrome P450 family and to the nucleotide sequences encoding them, and to their use in a method for the generation of pathogen-resistant plants.

REFERENCES:
patent: WO 01/38484 (2001-05-01), None
patent: WO 02/29018 (2002-04-01), None
W. Song, et al., “Molecular Cloning of an Allene Oxide Synthase: A Cytochrome P450 Specialized for the Metabolism of Fatty Acid Hydroperoxides,”Proc. Natl. Acad. Sci. USA,vol. 90, pp. 3519-8423, Sep. 1993.
K. Harms, et al., “Expression of a Flax Allene Oxide Synthase cDNA Leads to Increased Endogenous Jasmonic Acid (JA) Levels in Transgenic Potato Plants but Not to a Corresponding Activation of JA-Responding Genes,”The Plant Cell, vol. 7, pp. 1645-1654, Oct. 1995.
C. Wang, et al., “Overexpression of a Cytoplasm-Localized Allene Oxide Synthase Promotes the Wound-Induced Accumulation of Jasmonic Acid in Transgenic Tobacco,”Plant Molecular Biology,vol. 40, pp. 783-793, 1999.
Z. Pan, et al., “The Major Protein of Guayule Rubber Particles is a Cytochrome P450: Characterization Based on cDNA Cloning and Spectroscopic Analysis of the Solubilized Enzyme and Its Reaction Products,”The Journal of Biological Chemistry,vol. 270, No. 15, pp. 8487-8494, 1995.
GenBank accession No. AJ271093, created Apr. 16, 2000.
H. Maucher, et al., “Allene Oxide Synthases of Barley (Hordeum Vulgare cv. Salome): Tissue Specific Regulation in Seedling Development,”The Plant Journal,vol. 21, No. 2, pp. 199-213, 2000.
A. Itoh, et al., “Molecular Cloning of a Divinyl Ether Synthase: Identification as a CYP74 Cytochrome P-450,”The Journal of Biological Chemistry,vol. 276, No. 5, pp. 3620-3267, 2000.
GenBank accession No. AJ309541, created Feb. 3, 2001.
M. Stumpe, et al., “A Pathogen-Inducible Divinyl Ether Synthase (CYP74D) from Elicitor-Treated Potato Suspension Cells,”FEBS Letters,vol. 507, pp. 371-376, 2001.
T. K. Zank, et al., “Cloning and Functional Expression of the First Plant Fatty Acid Elongase Specific for Δ6-Polyunsaturated Fatty Acids,”Biochemical SocietyTransactions,vol. 28, Part 6, pp. 654-658, 2000.
T. Girke, et al., “Identification of a Novel Δ6-Acyl-Group Desaturase by Targeted Gene Disruption in Physcomitrella Patens,”The Plant Journal,vol. 15, No. 1, pp. 39-48, 1998.

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