Isolated DNA encoding the SPHI restriction endonuclease and rela

Details Isolated DNA encoding the SPHI restriction endonuclease and rela Isolated DNA encoding the SPHI restriction endonuclease and rela

1992-08-20

1993-11-16

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4353201, 43525233, 43525235, 435193, 536 232, C12N 922, C12N 1555

Patent

active

052623181

ABSTRACT:
The present invention is directed to a method for cloning and producing the SphI restriction endonuclease by 1) introducing the restriction endonuclease gene from Streptomyces phaeochromogenes into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the SphI restriction endonuclease activity, and 3) purifying the SphI restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the SphI restriction endonuclease activity.

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