Introduction of DNA into bacillus strains by conjugation

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4351721, 43525231, 4353201, C12P 2101, C15N 121, C15N 1564, C15N 1575

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active

058437207

ABSTRACT:
The present invention relates to methods of constructing recombinant Bacillus strains encoding a polypeptide of interest, in which method a DNA construct comprising a DNA sequence encoding the polypeptide of interest is introduced into a recipient Bacillus cell by conjugation from a donor cell.

REFERENCES:
patent: 5346818 (1994-09-01), Schafer et al.
Selinger et al., "Mobilization of Closely Related Plasmids pUB110 And pBC16 by Bacillus Plasmid pX0503 Requires Trans-Acting Open Reading Frame .beta.", Journal of Bacteriology, Jun. 1990, vol. 172. No. 6, pp. 3290-3297.

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