Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-08-26
1999-07-20
Degen, Nancy
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 29, C12Q 168, C12Q 102
Patent
active
059255234
ABSTRACT:
The present invention makes available an interaction trap system (hereinafter "ITS") which is derived using recombinantly engineered prokaryotic cells.
REFERENCES:
patent: 5283173 (1994-02-01), Fields et al.
Stanley Fields and Song, "A novel genetic system to detect protein-protein interactions",Nature(1989), 340: 245-246, Jul., 20, 1989.
Roger Brent et al,"A Eukaryotic transcriptional activator bearing the DNA specificity of a Prokaryotic repressor", Cell (1985) 43: 729-736, Dec. 1985.
Ma et al, "Converting a Eukaryotic transcriptional inhibitor into activator",CELL(1988),55: 443-446, Nov., 4, 1988.
Chien et Al. "The two-hybrid system: A method to identify and clone genes for protein that interact with a protein of interest",PNAS,(1991)88:9578-82, Nov. 1991.
Durfee et al. "The retinoblastoma protein associates with the protein phosphatase type 1 catalyitic subunit". Genes & Development (1993) 7:555-69, Dec. 18, 1992.
Gyuris et al. "Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2",Cell (1993),75:791-803, Nov. 19, 1993.
Vojtek et al."Mammalian Ras interacts directly with the Serine/Threonine kinase Raf.";Cell(1993), 74: 205-214, Jul. 16, 1993.
Golemis et al. "Fused Protein Domains inhibit DNA Binding by LexA", Mol. Cell. Biol. (1992),12:3006-3014, Jul. 1992.
Ebina et al. "LexA Protein is a Repressor of the Colicin El Gene*", J.Biol. Chem. (1983),258:13258-13261,Nov.10, 1983.
Harper et al. "The p21 Cdk-interacting Protein Cip1 is a potent inhibitor of G1 Cyclin-Dependent Kinases." CELL19930,75:805-816, Nov. 19, 1993.
Silver et al. "Techniques for cloning cDNAs encoding interactive transcriptional regulatory proteins.", Mol. Biol. Rep.(1993), 17: 155-165, Oct. 5, 1992.
Yang et al. "A protein Kinase Substrate identified by the Two-Hybrid system.", Science (1992), 257:680-685,Jul. 31, 1992.
Luban et al. "Human immunodeficiency virus Type 1 Gag Protein binds to Cyclophilins A and B.", Cell (1993), 73:1067-1078.
Hardy et al. "A RAP1-interacting protein involved in transcriptional silencing and telomere length regulation." Genes Devel. (1992), 6:801-814.
Bartel et al. "Elimination of false positives that arise in using the Two-Hybrid system." Biotechniques (1993), 14: 920-924.
Li et al. "Identification of mutations in p53 that affect its binding to SV40 large T antigen by using the yeast two-hybrid system." FASEB J. (1993), 7:957-963.
Lalo et al. "Interactions betweeen three common subunits of yeast RNA polymerases I and III." PNAS (1993), 90:5524-5528, Feb. 8, 1993.
Jackson et al. "Cell cycle regulation of the yeast Cdc7 Protein Kinase by Association with the Dbf4 protein." Mol. Cell. Biol. (1993), 13:2899-2908, May 1993.
Madura et al. "N-recognin/Ubc2 Interactions in the N-end Rule Pathway*." J.Biol. Chem. (1993),268:12046-12054, Jun. 5, 1993.
Iwabuchi et al. "Use of the two-hybrid system to identify the domain of p53 involved in oligomerization." Oncogene (1993), 8:1693-1696.
Ebright et al . "The Escherichia coli RNA polymerase alpha subunit: structure and function." Curr Opin Genet (1995), 5: 197-203.
Himmelfarb et al. "GAL11P : A yeast Mutation that potentiates the effect of weak GAL4-Derived activators." CELL (1990), 63: 1299-1309.
Severino et al. "Assembly of functional Escherichia coli RNA polymerase containing .beta. (beta) subunit fragments." Proceedings of the national Academy of Science (1995), 92:4591-4595.
Gentry et al. "The cloning and sequence of the gene encoding the omega subunit of Escherichia coli RNA polymerase." Gene (1986), 48:33-40.
Deora et al. "Purification and Characterization of DNA dependent RNA Polymerase from staphylococcus aureus." Biochem Biophys Res Commun (1995), 208: 610-616.
Barberis et al. "Contact with a component of a polymerase II Holoenzyme Suffices for gene Activation", Cell, vol.81,1995, pp. 359-368.
Chatterjee and Struhl: "Connecting a promoter-bound protein to TBP bypasses the need for a transcriptional activation domain", Nature,vol. 374, 1995, pp. 820-822.
Ishihama: "Protein-protein communication within the transcription apparatus", Journal of Bacteriology, vol. 175, No 9, 1993, pp. 2483-2489.
Dove et al: "Activation of Prokaryotic transcription through arbitrary protein-protein contacts", Nature, vol. 386, 1997, pp. 627-630.
Jacob et al. (1993) Construction of chimeric proteins from the sigma N-associated transcriptional activators VnfA and AnfA of Azotobacter vinelandii shows that the determinants of promoter specificity lie outside the `recognition` helix of the HTH motif i, 1993.
Dove Simon
Hochschild Ann
Joung J. Keith
Arnold Beth E.
Degen Nancy
President & Fellows of Harvard College
Schwartzman Robert
Vincent Matthew P.
LandOfFree
Intraction trap assay, reagents and uses thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Intraction trap assay, reagents and uses thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Intraction trap assay, reagents and uses thereof will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1321321