Instrument for determination of the base sequence of nucleic aci

Details Instrument for determination of the base sequence of nucleic aci Instrument for determination of the base sequence of nucleic aci

1987-12-04

1990-02-27

Niebling, John F.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

2041828, C25B 700

Patent

active

049043668

ABSTRACT:
In the instrument for determination of the base sequence of the present invention, the ionic strength of a buffer solution in a gel is made lower at a detection part than in a region from the detection part towards a negative electrode. Therefore, at the detection part, the electric field intensity can be increased, resulting in a higher migration speed, and hence the electrophoretic pattern can be extended, so that the distance between two adjacent electrophoretic bands can be elongated.
Consequently, according to the present invention, the slit width can be narrowed relatively to the electrophoretic pattern without actually narrowing the slit width, and the resolving power can be enhanced without lowering the detection sensitivity.

REFERENCES:
patent: 4707235 (1987-11-01), Englert
Biggin et al., "Buffer Gradient Gels and .sup.35 S Label as an Aid to Rapid DNA Sequence Determination", Proc. Nat. Acad. Sci. USA, vol. 80, pp. 3963-3965, (1983).
Olsson et al., "Uniformly Spaced Banding Pattern in DNA Sequencing Gels by Use of Field--Strength Gradient", Journal of Biochemistry and Biophysical Methods, vol. 10, p. 84, (1984).

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