Chemistry: analytical and immunological testing – Involving kinetic measurement of antigen-antibody reaction
Patent
1992-07-17
1994-12-06
Nucker, Christine M.
Chemistry: analytical and immunological testing
Involving kinetic measurement of antigen-antibody reaction
435 71, G01N 33557
Patent
active
053710215
ABSTRACT:
An initial rate photometric immunoassay method useful for detecting and quantifying analytes in various physiological fluids is disclosed. The method is carried out by combining in a liquid medium an undiluted sample of analyte-containing physiological fluid, such as serum, and an excess of an anti-analyte antibody. A substantially constant initial rate of increase of the liquid medium's turbidity, due to the resulting immunoprecipitation reaction, is measured in real time and compared to a calibration curve prepared from known analyte concentrations to detect and quantify the analyte. Detection and quantification of numerous analytes including, haptens, drugs and proteins, in a wide variety of physiological fluids can be rapidly carried out by the present method.
REFERENCES:
patent: 4204837 (1980-05-01), Sternberg et al.
patent: 4205954 (1980-06-01), Babson
patent: 4268171 (1981-05-01), Sternberg
patent: 4305665 (1981-12-01), Achter et al.
patent: 4313929 (1982-02-01), Morita et al.
patent: 4446239 (1984-05-01), Tsuji et al.
patent: 4521521 (1985-06-01), Abbott et al.
patent: 4554257 (1985-11-01), Aladjem et al.
patent: 4604365 (1986-08-01), O'Neill et al.
patent: 4766083 (1988-08-01), Miyashita et al.
patent: 4835110 (1989-05-01), Seymour et al.
patent: 5162236 (1992-11-01), Pang et al.
Rose et al, editors; Manual of Clinical Laboratory Immunology, 3rd ed, Wash. D.C. 1986, Ch 5, pp. 25-32.
Finley et al; Clin Chem 2713, 405-409 (1981).
Manual of Clinical Laboratory Immunology, 3rd Ed., 1986 Chapter 6, Sternberg, pp. 33-37.
Kearne Elizabeth K.
Oh Chan S.
Beckman Instruments Inc.
Donovan Stephen
Grant Arnold
May William H.
Nucker Christine M.
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