In vivo incorporation of unnatural amino acids

Details In vivo incorporation of unnatural amino acids In vivo incorporation of unnatural amino acids

2008-05-06

2008-05-06

Prouty, Rebecca E. (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S193000, C435S091400, C435S252330, C435S320100, C435S015000, C435S069100, C536S023200

Reexamination Certificate

active

07368275

ABSTRACT:
The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.

REFERENCES:
patent: 5370995 (1994-12-01), Hennecke et al.
patent: 7045337 (2006-05-01), Schultz et al.
patent: 7083970 (2006-08-01), Schultz et al.
Kowal et al Twenty-first amino-acyl tRNA synthetase suppressor tRNA pairs for possible use in site specific incorporation of amino acid analogues into proteins in eukaryotes and eubacteria. PNASFeb. 27, 2001, vol. 98, No. 5, pp. 2268-2273.
Anderson et al., Exploring the Limits of Codon and Anticodon Size,Chemistry and Biology, vol. 9, 237-244 (2002).
Azoulay et al., Glutamine analogues as Potential Antimalarials,Eur. J. Med. Chem. 26, 201-5 (1991).
Bain et al., Biosynthetic site-specific Incorporation of a non-natural amino acid into a polypeptide,J. Am. Chem. Soc., 111:8013-8014 (1989).
Barton et al., Synthesis of Novel a-Amino-Acids and Derivatives Using Radical Chemistry: Synthesis of L- and D-a-Amino-Adipic Acids, L-a,-aminopimelic Acid and Appropriate Unsaturated Derivatives.Tetrahedron Lett. 43, 4297-4308 (1987).
Boles et al.,Nat. Struct. Biol., 1:283 (1994).
Bradley et al., tRNA2GIn Su+2 mutants that increase amber suppression,J. Bacteriol. 145:704-712 (1981).
Brick et al.,J. Mol. Biol., 208:83-98 (1988).
Brunner, New Photolabeling and crosslinking methods,Annu. Rev. Biochem., 62:483-514 (1993).
Budisa et al.,Eur. J. Biochem., 230:788 (1995).
Budisa et al.,Faseb J. 13:41-51 (1999).
Budisa et al.,J. Mol. Biol., 270:616 (1997).
Budisa et al.,Proc. Natl. Acad. Sci. U S A, 95:455 (1998).
Christie & Rapoport, Synthesis of Optically Pure Pipecolates from L-Asparagine. Application to the Total Synthesis of (+)-Apovincamine through Amino Acid Decarbonylation and Iminium Ion Cyclization.J. Org. Chem. 50:1239-1246 (1985).
Cornish et al.,Angew. Chem. Int. Ed. Engl., 34:621 (1995).
Cornish et al.,J. Am. Chem. Soc., 118:8150-8151 (1996).
Craig et al., Absolute Configuration of the Enantiomers of 7-Chloro-4 [[4-(diethylamino)-1-methylbutyl]amino]quinoline (Chloroquine).J. Org. Chem. 53, 1167-1170 (1988).
Database NCBI, GenBank Accession No. E64348, Bult et al. ‘Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii,’ Gene Sequence, Jun. 3, 1996.
Doctor & Mudd,J. Biol. Chem., 238:3677 (1963).
Doring et al.,Science, 292:501 (2001).
Dougherty,Curr. Opin. Chem. Biol., 4:645 (2000).
Duewel et al.,Biochemistry, 36:3404 (1997).
Dunten & Mowbray, Crystal structure of the dipeptide binding protein fromEscherichia coliinvolved in active transport and chemotaxis.Protein Science4, 2327-34 (1995).
Ellman et al., Biosynthetic method for introducing unnatural amino acids site-specifically into proteins,Methods in Enz., 202:301-336 (1992).
Ellman et al., Site-specific incorporation of novel backbone structures into proteins,Science, 255:197-200 (1992).
England et al.,Cell, 96:89 (1999).
Fechter et al., Major tyrosine identity determinants inMethanococcus jannaschiiandSaccharomyces cerevisiaetRNA Tyr are conserved but expressed differently,Eur. J. Biochem. 268:761-767 (2001).
Francisco et al., Production and fluorescence-activated cell sorting ofEscherichia coliexpressing a functional antibody fragment on the external surface.Proc Natl Acad SciU S A. 90:10444-8 (1993).
Francklyn et al. (2002) “Aminoacyl-tRNA synthetases: Versatile players in the changing theater of translation.”RNA, 8:1363-1372.
Friedman & Chatterrji, Synthesis of Derivatives of Glutamine as Model Substrates for Anti-Tumor Agents.J. Am. Chem. Soc. 81, 3750-3752 (1959).
Furter,Protein Sci., 7:419 (1998).
Gabriel & McClain, A set of plasmids constitutively producing different RNA levels inEscherichia coli,J. Mol. Biol. 290 (1999) 385-389.
Gallivan et al.,Chem. Biol., 4:739 (1997).
GenBank Accession No. Q57834, publicly available Nov. 1, 1997.
Giegé et al.,Biochimie, 78:605 (1996).
Giegé et al., Universal rules and idiosyncratic features in tRNA identity,Nucleic Acids Res. 26:5017-5035 (1998).
Gay et al.,FEBS Lett. 318:167 (1993).
Gucklan et al.,Angew. Chem. Int. Ed. Engl., 36, 2825 (1997).
Hamano-Takaku et al.,J. Biol. Chem., 275:40324 (2000).
Hartley et al., Expression of its cloned inhibitor permits expression of a cloned ribonuclease,J. Mol. Biol. 202:913-915 (1988).
He, et al.,Microbiology, 147:2817-2829 (2001).
Hendrickson et al.,Embo J., 9:1665 (1990).
Hirao, et al., An unnatural base pair for incorporating amino acid analogues into protein,Nature Biotechnology, 20:177-182 (2002).
Hohsaka et al.,J. Am. Chem. Soc., 121:34 (1999).
Ibba & Hennecke,FEBS Lett., 362:272 (1995).
Ibba et al.,Biochemistry, 33:7107 (1994).
Ibba et al., “Strategies for in vitro and in vivo incorporation translation with non-natural amino acids”Biotechnol. Genet. Eng. Rev. 13:197-216.
Jakubowski & Goldman,Microbiol. Rev., 56:412 (1992).
Jeruzalmi & Steitz,Embo J., 17, 4101-4113 (1998).
Jacovic & Hartley, Protein-protein interaction: a genetic selection for compensating mutations at the barnase-barstar interface.Proceedings of the National Academy of Sciences of theUnited States of America, 93: 2343-2347 (1996).
Kiga et al. (2002) “An engineeredEscherichia colityrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system.”PNAS, vol. 99, No. 15, pp. 9715-9723.
Klick & Tirrell,Tetrahedron, 56:9487 (2000).
Escherichia coli,J. Mol. Biol.290 (1999) 385-389.
Ibba (1996) “Strategies for in vitro and in vivo incorporation translation with non-natural amino acids”Biotechnol. Genet. Eng. Rev.13:197-216.
King et al., A New Synthesis of Glutamine and of γ-Dipeptides of Glutamic Acid from Phthylated Intermediates.J. Chem. Soc., 4:3315-3319 (1949).
Kleeman et al.,J. Biol. Chem., 272:14420 (1997).
Kleina et al., Construction ofEscherichia coliamber suppressor tRNA genes. II. Synthesis of additional tRNA genes and improvement of suppressor efficiency,J. Mol. Biol. 213:705-717 (1990).
Kool,Curr. Opin. Chem. Biol., 4:602 (2000).
Koskinen & Rapoport, Synthesis of 4-Substituted Prolines as Conformationally Constrained Amino Acid Analogues.J. Org. Chem. 54, 1859-1866. (1989).
Kowal & Oliver,Nucl. Acid. Res., 25:4685 (1997).
Kowal et al.,Proc. Natl. Acad. Sci. U S A, 98:2268 (2001).
Krieg et al., Photocrosslinking of the signal sequence of nascent preprolactin of the 54-kilodalton polypeptide of the signal recognition particle,Proc. Natl. Acad. Sci, 83(22):8604-8608 (1986).
Kwok & Wong,Can. J. Biochem., 58:213 (1980).
Lee et al.,Biotechnology Letters, 20:479-482, (1998).
Liu & Schultz, Progress toward the evolution of an organism with an expanded genetic code,Proc. Natl. Acad. Sci. USA 96:4780-4785 (1999).
Liu et al.,Chem. Biol., 4:685 (1997).
Liu et al., Engineering a tRNA and aminoacyl-tRNA synthetase for the site-specific incorporation of unnatural amino acids into proteins in vivo,Proc. Natl. Acad. Sci. USA 94:10091-10097 (1997).
Lorincz et al.,Cytometry, 24, 321-329 (1996).
Lu et al.,Nat. Neurosci., 4:239 (2001).
Ma et al.,Biochemistry, 32:7939 (1993).
Magliery, Expanding the Genetic Code: Selection of Efficient Suppressors of Four-base Codons and Identification of “Shifty” Four-base Codons with a Library Approach inEscherichia coli,J. Mol. Biol. 307: 755-769 (2001).
Matsoukas et al.,J. Med. Chem., 38: 4660-4669 (1995).
McMinn et al.,J. Am. Chem. Soc., 121:11586 (1999).
Meggers et al.,J. Am. Chem. Soc., 122:10714 (2000).
Mendel et al., SIte-Directed Mutagenesis with an Expanded Genetic Code,Annu. Rev. Biophys. Biomol. Struct. 24, 435-62 (1995).
Miller et al.,Neuron, 20:619 (1998).
Minks et al.,Anal. Biochem., 284:29 (2000).
Moore et al.,J. Mol. Biol., 298:195 (2000).
Nagagawa et al. (2000) “Mutational Analysis of Invariant Valine B12 in Insulin: Implications for Receptor Binding.”Biochemistry, 39:15826-15835.
Nichitenko et al., A structur

Affiliated with

Also associated with

Rating

In vivo incorporation of unnatural amino acids does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with In vivo incorporation of unnatural amino acids, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and In vivo incorporation of unnatural amino acids will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2770353