In vitro germinal centers

Details In vitro germinal centers In vitro germinal centers

2011-08-23

2011-08-23

Belyavskyi, Michail (Department: 1644)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of co-culturing cells

C435S375000, C435S377000

Reexamination Certificate

active

08003387

ABSTRACT:
The present invention incorporates germinal centers (GCs) into three-dimensional (3D) engineered tissue constructs (ETCs). In an embodiment, we have incorporated the GC in the design of an artificial immune system (AIS) to examine immune responses to vaccines and other compounds. Development of an in vitro GC adds functionality to an AIS, in that it enables generation of an in vitro human humoral response by human B lymphocytes that is accurate and reproducible, without using human subjects. The invention also permits evaluation of, for example, vaccines, allergens, and immunogens, and activation of human B cells specific for a given antigen, which can then be used to generate human antibodies. In an embodiment of the present invention the function of the in vitro GC is enhanced by placing FDCs and other immune cells in a 3D ETC; FDCs appear more effective over a longer time (antibody production is sustained for up to about 14 days.

REFERENCES:
patent: 5008116 (1991-04-01), Cahn
patent: 5160490 (1992-11-01), Naughton et al.
patent: 5354686 (1994-10-01), Haberman
patent: 5562910 (1996-10-01), Daynes et al.
patent: 5725856 (1998-03-01), Hudziak et al.
patent: 5739001 (1998-04-01), Brown et al.
patent: 5750329 (1998-05-01), Quinn et al.
patent: 5785964 (1998-07-01), Naughton et al.
patent: 6143501 (2000-11-01), Sittinger et al.
patent: 6177282 (2001-01-01), McIntyre
patent: 6274378 (2001-08-01), Steinman et al.
patent: 6479064 (2002-11-01), Atala
patent: 6541225 (2003-04-01), Li
patent: 6835550 (2004-12-01), Estell et al.
patent: 2002/0155108 (2002-10-01), Barbera-Guillem et al.
patent: 2003/0109042 (2003-06-01), Wu et al.
patent: 2003/0147923 (2003-08-01), Klaviniskis
patent: 2003/0199006 (2003-10-01), Britz et al.
patent: 2003/0207287 (2003-11-01), Short
patent: 2004/0009943 (2004-01-01), Semple et al.
patent: 2004/0109876 (2004-06-01), Yamamoto et al.
patent: 2004/0234510 (2004-11-01), Mochitate
patent: 2005/0191743 (2005-09-01), Wu et al.
patent: 2005/0229264 (2005-10-01), Chang et al.
patent: 2005/0282148 (2005-12-01), Warren et al.
patent: 2006/0078540 (2006-04-01), Warren et al.
patent: 2006/0105454 (2006-05-01), Son et al.
patent: 2006/0270029 (2006-11-01), Warren et al.
patent: 2006/0275270 (2006-12-01), Warren et al.
patent: 2007/0015136 (2007-01-01), Sanchez-Schmitz et al.
patent: 2007/0141552 (2007-06-01), Warren et al.
patent: 2007/0154956 (2007-07-01), Warren et al.
patent: 2007/0178076 (2007-08-01), Drake et al.
patent: 2007/0218054 (2007-09-01), Sukumar et al.
patent: 2008/0008653 (2008-01-01), Tew et al.
patent: 2009/0011455 (2009-01-01), Warren et al.
patent: 2009/0104221 (2009-04-01), El Shikh et al.
patent: 2009/0117581 (2009-05-01), Warren et al.
patent: 0 358 506 (1989-09-01), None
patent: 1013668 (2000-06-01), None
patent: 1 437 147 (2002-09-01), None
patent: 1970444 (2006-12-01), None
patent: 99/12972 (1999-03-01), None
patent: WO 99/15629 (1999-04-01), None
patent: WO 99/43788 (1999-09-01), None
patent: WO99/49319 (1999-09-01), None
patent: WO 03/041568 (2003-05-01), None
patent: WO 03/050271 (2003-06-01), None
patent: WO 2004/031361 (2004-04-01), None
patent: WO2004/101773 (2004-11-01), None
patent: 2005/013896 (2005-02-01), None
patent: 2005/072088 (2005-08-01), None
patent: WO 2005/104755 (2005-11-01), None
patent: WO 2007/075979 (2007-07-01), None
patent: 2007/108835 (2007-09-01), None
patent: WO 2007/106559 (2007-09-01), None
patent: WO 2007/146267 (2007-12-01), None
Caux et al, Res. Immunol., 1994, v. 145, pp. 235-239.
Grouard et al., Current Topic in Microbiology and Immunology, 1995, v.201, pp. 105-117.
International Search Report—PCT/US08/69172, 2008.
International Search Report—PCT/US08/70107, 2008.
Dubois et al., J. Leukocyte Biology, 1999, v.66, p. 224-230.
Gansuvd et al., Human Immunol., 2003, v.64, p. 427-439.
Clayton et al., Clin. Exp. Immunol., 2003, v.132, p. 174-179.
D'Amico et al., Blood 92:207-214 (1998).
Simmingskoeld et al., Scand. J. Immunol. 7:233-238 (1978).
Transwell® Permeable Supports Selection and Use Guide, Corning Corp., pp. 1-12 (2009).
Khademhosseini et al., “Microscale Technologies for Tissue Engineering and Biology,” Proc. Natl. Acad. Sci. USA, vol. 103, pp. 2480-2487 (2006).
Suematsu, et al., “Generation of a Synthetic Lymphoid Tissue-Like Organoid in Mice”, Nature Biotechnology, vol. 22, No. 12, pp. 1539-1545 (Dec. 2004).
Randolph, et al., “Role of Tissue Factor in Adhesion of Mononuclear Phagocytes to and Trafficking Through Endothelium in Vitro”, Blood, vol. 92, pp. 4167-4177 (1998).
Randolph, et al., “Differentiation of Monocytes into Dendritic Cells in a Model of Transendothelial Trafficking”, Science, vol. 282, pp. 480-483 (1998).
Randolph, et al., “A Physiologic Function for p-Glycoprotein (MDR-1) During the Migration of Dendritic Cells from Skin Via Afferent Lymphatic Vessels”, Proc. Natl. Acad. Sci., vol. 95, pp. 6924-2929 (1998).
Randolph, et al., “The CD16(+) (FcγRIII(+)) Subset of Human Monocytes Preferentially Becomes Migratory Dendritic Cells in a Model Tissue Setting”, J. Exp. Med., vol. 196, No. 4, pp. 517-527 (2002).
Randolph, et al., “Mononuclear Phagocytes Egress from an In Vitro Model of the Vascular Wall by Migrating Across Endothelium in the Basal to Apical Direction: Role of Intercellular Adhesion Molecule 1 and the CD11/CD18 Integrins”, J. Exp. Med., vol. 183, pp. 451-462 (1996).
Randolph, et al., “A Soluble Gradient of Endogenous Monocyte Chemoattractant Protein-1 Promotes the Transendothelial Migration of Monocytes In Vitro”, The Journal of Immunology, vol. 155, pp. 3610-3618 (1995).
Furie, et al., “Migration of Neutrophils Across Endothelial Monolayers is Stimulated by Treatment of the Monolayers with Interleukin-1 or Tumor Necrosis Factor-Alpha”, The Journal of Immunology, vol. 143, pp. 3309-3317 (1989).
Robbiani, et al., “The Leukotriene C4 Transporter MRP1 Regulates CCL19 (MIP-3β, ELC)-Dependent Mobilization of Dendritic Cells to Lymph Nodes”, Cell, vol. 103, pp. 757-768 (2000).
Podgrabinska, et al., “Molecular Characterization of Lymphatic Endothelial Cells”, Proc. Natl. Acad. Sci. U.S.A., vol. 99, No. 25, pp. 16069-16074 (2002).
Berman, et al., “Roles of Platelet/Endothelial Cell Adhesion Molecule-1 (PECAM-1, CD31) in Natural Killer Cell Transendothelial Migration and Beta 2 Integrin Activation”, The Journal of Immunology, vol. 156, pp. 1515-1524 (1996).
Gergel, et al., “Activation of Endothelium byBorrelia burgdorferiIn Vitro Enhances Transmigration of Specific Subsets of T Lymphocytes”, Infection and Immunity, vol. 69, pp. 2190-2197 (2001).
Qu, et al., “Autocrine Type I IFN and Contact with Endothelium Promote the Presentation of Influenza A Virus by Monocyte-Derived APC”, The Journal of Immunology, vol. 170, pp. 1010-1018 (2003).
Mellman, et al.. “Dendritic Cells: Specialized and Regulated Antigen Processing Machines”, Cell, vol. 106, pp. 255-258 (2001).
Lukas, et al., “Human Cutaneous Dendritic Cells Migrate Through Dermal Lymphatic Vessels in a Skin Organ Culture Model”, The Journal of Investigative Dermatology, vol. 106, pp. 1293-1299 (1996).
Fransson, et al., “Culture of Human Epidermal Langerhans Cells in a Skin Equivalent”, British Journal of Dermatology, vol. 139, pp. 598-604 (1998).
Banchereau, et al., “Immunobiology of Dendritic Cells”, Annu. Rev. Immunol., vol. 18, pp. 767-811 (2000).
Butcher, et al., “Lymphocyte Trafficking and Regional Immunity”, Advances in Immunology, vol. 72, pp. 209-253 (1999).
Sprent, et al., “Antigen-Induced Selective Recruitment of Circulating Lymphocytes”, Cellular Immunology, vol. 2, pp. 171-181 (1971).
Garside, et al., “Visualization of Specific B and T Lumphocyte Interactions in the Lymph Node”, Science, vol. 281, pp. 96-99 (1998).
Jenkins, et al., “In Vivo Activation of Antigen-Specific CD4 T Cells”, Annu. Rev.

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