Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...
Patent
1997-10-20
1999-11-30
Robinson, Douglas W.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
4352522, 435419, 4351723, 800278, 800314, C12N 1582, C12N 1584, A01H 400
Patent
active
059946245
ABSTRACT:
The present invention relates to a method for producing a transgenic plant in which a transforming agent such as a recombinant Agrobacterium or an isolated DNA molecule capable of conferring a desired phenotypic trait is injected into plant tissues using a needleless injection device. A preferred embodiment of the method provides the precise delivery of the transforming agent to floral tissues of a plant, allowing the direct insertion of the DNA of the transforming agent into germline cells of the plant that develop into seeds. This embodiment provides a more efficient method of transformation and subsequent regeneration of a transgenic plant.
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Koonce Linda
Trolinder Norma L.
Cotton Incorporated
Robinson Douglas W.
Zaghmout Ousama
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