Immunofixation electrophoresis process

Details Immunofixation electrophoresis process Immunofixation electrophoresis process

1984-03-16

1987-05-26

Niebling, John F.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

436516, B01K 500

Patent

active

046683631

ABSTRACT:
A method comprising (a) applying a sample to at least two application areas on an electrophoretic gel; (b) electrophoresing the gel; (c) aligning a template onto the electrophoresed gel, the template having a template slot corresponding to each electrophoresed area; (d) applying a composition capable of fixing proteins in situ to at least one template slot and applying an antisera capable of reacting with one protein to at least one of the remaining template slots; (e) incubating the resultant product of step (d); (f) removing the template from the incubated, electrophoresed gel; (g) washing the incubated electrophoresed gel of step (f); (h) drying the washed gel of step (g); (i) staining the dryed gel of step (h); (j) destaining the stained gel of step (i); (k) drying the destained gel of step (j); and (l) analyzing the dryed gel of step (k).

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