Immunochemical measuring method using second antigenic substance

Details Immunochemical measuring method using second antigenic substance Immunochemical measuring method using second antigenic substance

1977-10-03

1980-01-22

Sebastian, Leland A.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

23230B, 232303, 232306, 23DIG16, 435 7, 424 12, 23DIG21, A61K 2704

Patent

active

041850840

ABSTRACT:
An immunochemical measuring method using a second antigenic substance and a labeled antibody to the second antigenic substance.
An unknown amount of an antigen (Ag 1) to be measured and a given amount of a conjugate (Ag 1-Ag 2) coupled a second antigen (Ag 2) to the Ag 1 are reacted with a given amount of an antibody (Ab 1) to the Ag 1. Ag 1-Ag 2 bind to the Ab 1 in proportion to their respective amount, forming an Ab 1-Ag 1-Ag 2 complex. Then, by reaction of the complex with a labeled antibody (labeled Ab 2) to the Ag 2, Ab 1-Ag 1-Ag 2-labeled Ab 2 is formed. It is possible to estimate the amount of Ag 1 to be measured by determining the activity of labeled Ab 2 attached to the complex.

REFERENCES:
patent: 3935074 (1976-01-01), Rubenstein et al.
patent: 3940475 (1976-02-01), Gross
patent: 3949064 (1976-04-01), Bornstein et al.

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