Textiles: fiber preparation
Patent
1977-09-28
1979-02-20
Wiseman, Thomas G.
Textiles: fiber preparation
23230B, 1951035M, 424 12, C17K 104
Patent
active
041405811
ABSTRACT:
This invention relates to means for determining the presence of Neisseria bacteria in a fluid sample, the method generally comprising reacting antibodies with an enzyme released during lysis of the fluid sample, and then testing for inhibition of activity of the enzyme. Inhibition of enzyme activity caused through the contact of antibodies specific to the enzyme can be readily detected. The enzyme released from Neisseria bacteria upon lysis possesses the faculty of oxidizing 1,2-propanediol and reducing nicotinamide-adenine-dinucleotide (NAD). Although the structure and behavior of the enzyme are not comprehended entirely, the above-mentioned faculty of the material has suggested the term 1,2-propanediol dehydrogenase therefor.
The specific inventive feature of the instant method is the use of (NH.sub.4).sub.2 SO.sub.4 to precipitate the antigen-antibody complex which thereby concentrates the enzymes and can remove possibly interfering materials.
REFERENCES:
patent: 3773625 (1973-11-01), Sternberger et al.
patent: 3875011 (1975-04-01), Rubenstein et al.
patent: 3974269 (1976-08-01), Maley
patent: 4029756 (1977-06-01), Gaafan
Corning Glass Works
Janes Jr. Clinton S.
Maycock William E.
Patty, Jr. Clarence R.
Wiseman Thomas G.
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