Immunoassay method and immunoassay reagent kit to be used...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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Details

C435S007920, C436S501000, C436S164000

Reexamination Certificate

active

07056682

ABSTRACT:
In step St1shown in FIG.1, a reaction system including a sample for which the content of a subject substance is to be measured, a specific-binding substance specifically binding to the subject substance, and phthalic acid or phthalate salt is constructed. The pH of the reaction system is set at less than 7. When the subject substance is an antigen, the specific-binding substance is an antibody. In reverse, when the subject substance is an antibody, the specific-binding substance is an antigen. In step St2in FIG.1, an optical property of the reaction system is measured. When agglutinate complexes are produced in the step St1, the reaction system becomes turbid, causing a change in scattered light intensity, transmitted light amount and the like. Therefore, by measuring the scattered light intensity, the transmitted light amount or the like, the degree of turbidity of the reaction system can be estimated.

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Takahiro Hamasaki, “Edta Sonzaiji No Shihan Kokessei No Crp Eno Ketsugo Kassei No Teika”, The Japanese Journal of Clinical Pathology, Feb. 23, 1984, vol. 32, No. 2, pp. 223 to 224.

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