Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1984-10-04
1987-11-17
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4351723, 435948, 43524026, 935 57, 935 71, C12N 500, C12N 1500, C12R 191
Patent
active
047074485
ABSTRACT:
The present invention discloses permanent establishment of an immortal line of fetal glial cells. The fetal glial cell line is capable of mitotically proliferating and continually growing in vitro under suitable culture media and environmental conditions. A method of producing immortalized human fetal glial cell line is also disclosed. The method comprises transfecting primary human fetal glial cells with an origin-defective mutant of SV40 virus, passaging the resulting astroglial cells through suitable number of cycles and obtaining the desired cell line. The cell line of the present invention is capable of supporting multiplication of JC virus.
REFERENCES:
Brooks, S. E., Amsterdam, D., Hoffman, L. M., Adachi, M. and Scheck, L. "Cytology, Growth Characteristics . . . " Biological Abstracts 69:4077 (1979).
Hoffman, L. M., Brooks, S. E., and Schneck, L., "Human Fetal Brain Cells in Culture" Biochim Biophys Acta 665:359-361 (1981).
Gluzman, Y. "SV40-Transformed Simian Cells Support the Replication of Early SV40 Mutants" Cell 23:175-182 (1981).
Wroblewska, Z., Wellish, M. and Gilden, D., "Growth of JC Virus in Adult Human Brain Cell Cultures," Biological Abstracts 71:7071 (1981).
B. Lewin Genes John Wiley & Sons, New York, 1983, p. 684.
Brooks, S. E., D. Amsterdam, L. M. Hoffman, M. Adachi and B. Schneck Journal of Cell Science 38:211-223, 1979.
Major, E. O., A. E. Miller, P. Mourrain, R. G. Traub, E. DeWidt and J. Sever, Proc. Natl. Acad. Sci. U.S.A. 82:1257-1261, 1985.
Eugene Major, SV40, Polyoma and Adenoviruses; Aug. 15-19 1984; p. 160 (abstract of papers presented in 84 Tumor Virus Meeting).
Major et al., JC Virus-Induced Owl Monkey Gliblastoma Cells in Culture . . . ; 1984; pp. 359-367; Virology 136.
Major et al., Human Embryonic Kidney Cells: Stable Transfusion with an Origin-Defective Simian Virus 40 . . . ; Mol & Cell Bio, 1984, pp. 379-382.
Maurey Karen
The United States of America as represented by the Department of
Wiseman Thomas G.
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