Immobilized enzyme film, protein immobilized film and process fo

Details Immobilized enzyme film, protein immobilized film and process fo Immobilized enzyme film, protein immobilized film and process fo

1992-11-06

1994-10-18

Kight, III, John

Radiation imagery chemistry: process, composition, or product th

Imaging affecting physical property of radiation sensitive...

Making electrical device

435288, G03C 500

Patent

active

053567579

ABSTRACT:
Disclosed are an immobilized enzyme film, characterized in that said film is formed using an enzyme solution prepared by adding 1 to 3 parts by weight of a 50 to 100 wt % water-soluble crosslinking agent having at least two epoxy groups in the molecule and 1 to 3 parts by weight of a 1 to 2 wt % glutaraldehyde to 1 to 3 parts by weight of a 10 to 50 wt % aqueous protein solution containing an enzyme, and a process for forming the same on an ion-sensitive field effect transistor. The thus formed immobilized enzyme film is of an uniform thickness and stable by virtue of its increased hydrophilicity and higher elasticity, whereby deactivation of the enzyme to be caused by the shrinkage of the film can be prevented.

REFERENCES:
patent: 4795707 (1989-01-01), Niiyama et al.
patent: 4894339 (1990-01-01), Hanazato et al.

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