IL-21 production in prokaryotic hosts

Details IL-21 production in prokaryotic hosts IL-21 production in prokaryotic hosts

2007-07-31

2007-07-31

Nickol, Gary B. (Department: 1646)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S320100, C435S252300, C435S252800, C530S351000

Reexamination Certificate

active

10735149

ABSTRACT:
The expression vectors and methods using anE. coliexpression system for the large scale production of IL-21 are described. The vectors utilize the IL-21 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation inE. coli.Using the expression vectors, the IL-21 gene was produced inE. colito a level of greater than 1 g/L in fed batch fermentation. Also included are OmpT deficientE. colistrains transformed with an IL-21 expression vector.

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