Identification, characterization, and method of production of a

Details Identification, characterization, and method of production of a Identification, characterization, and method of production of a

1989-03-16

1991-11-05

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435198, 435271, 435814, C12N 120, C12N 920

Patent

active

050631608

ABSTRACT:
A novel lipase from a newly-discovered strain of Pseudomonas alcaligenes microorganism having (i) an optimum pH for activity of about 10.+-.0.5; (ii) an optimum temperature for activity of about 45.degree. to 55.degree. C.; (iii) an optimum pH for stability of about 7.0.+-.0.5; (iv) a molecular weight as measured by gel permeation chromotagraphy of about 8.8.times.10.sup.4 ; and (v) chemical stability for at least 30 days in the presence of the surfactants. Also claimed is a biologically pure culture of the microorganism, and a method for the production of the lipase.

REFERENCES:
patent: 3511753 (1970-05-01), Prave et al.
patent: 3674643 (1972-07-01), Aunstrup et al.
patent: 4283494 (1981-08-01), Kokusho et al.
patent: 4318818 (1982-04-01), Letton et al.
Watanabe, N. et al., (1977), Agric. Biol. Chem. 41(8), 1353-1358.
Gel Filtration Theory and Practice (1979), Pharmacia Fine Chemicals, p. 42.
Ralston-Barrett, E., N. J. Palleronie, and M. Doudoroff, Inter. J. System. Bacteriol, 26(4): 421-426 (1976).
ATCC Catalogue of Bacteria and Phages, (17th ed), 1989, p. 170.

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