Hydrolytic substrates for an analyte-dependent enzyme...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007400, C435S007720, C435S018000, C435S968000, C435S207000, C436S164000, C436S546000, C530S402000, C570S127000

Reexamination Certificate

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10560577

ABSTRACT:
Disclosed are compounds of the general formulas:wherein Y is a moiety capable of being cleaved by a hydrolytic enzyme; L is a detectable label; X is a linking group; Z is a halogen; and R is hydrogen, an alkyl, or a halogen. Compounds of this general formula can be used as substrates in an analyte-dependent enzyme activation system, such as that employed in connection with catalyzed reporter depositions. Also disclosed are assays using the compounds, and kits for carrying out the assays.

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Zhu, Qing, et al., Activity-based fluorescent probes that target phosphatases, 2003 Elsevier Science Ltd., Tetrahedron Letters 44 (2003) 2669-2672.
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