Human tPA production using vectors coding for DHFR protein

Details Human tPA production using vectors coding for DHFR protein Human tPA production using vectors coding for DHFR protein

1995-05-26

1998-12-15

Martinell, James

Chemistry: molecular biology and microbiology

Vector, per se

435358, 435359, C12N 1558

Patent

active

058495746

ABSTRACT:
A method for producing tissue plasminogen activator (t-PA) in eukaryotic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild type DHFR with methotrexate.

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