Human osteoclast-derived cathepsin

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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4352523, 43525411, 4353201, 536 232, 536 243, C12N 1557, C12N 1563, C12N 121, C12N 510

Patent

active

055019692

ABSTRACT:
Disclosed is a human osteoclast-derived cathepsin (Cathepsin O) polypeptide and DNA(RNA) encoding such cathepsin O polypeptides. Also provided is a procedure for producing such polypeptide by recombinant techniques. The present invention also discloses antibodies, antagonists and inhibitors of such polypeptide which may be used to prevent the action of such polypeptide and therefore may be used therapeutically to treat bone diseases such as osteoporosis and cancers, such as tumor metastases.

REFERENCES:
Lerner, V. H. and Grubb, A., Human Cystatin C, a Cysteine Proteinase Inhibitor, Inhibits Bone Resorption In Vitro Stimulated by Parathyroid Harmone and Parathyroid Hormone Related Peptide of Malignancy, Journal of Bone and Mineral Research, 7:433-439 (1992).
Smith, S. M. and Gottesmann, M. M., Activity and Deletion Analysis of Recombinant Human Cathepsin L Expressed in E. Coli J. Biol. Chem., 264:20487-20495 (1989).
Bromme, D. et al., Functional Expression of Human Cathepsins in Saccharomyces Cerevisiae, J. Biol. Chem., 268:4832-4838 (1993).
Kane, S. E. and Gottesmann, M. M., The role of Cathepsin L in Malignant Transformation, Seminars in Cancer Biology 1:127-136 (1990).
Sloane, B. F., Cathepsin B and Cystatins: Evidence for a Role in Cancer Progression, Seminars in Cancer Biology, 1:137-152 (1990).
Delaisse, J. M., The Effects of Inhibitors of Cysteine--Proteinases and Collagenase on the Resorptive Activity of Isolated Osteoclasts Bone, 8:305-313 (1987).
Tezuka, K. et al., Molecular Cloning of a Possible Cysteine J. Biology Chem. 269:1106-1109 (1994).
Hashimoto et al., World Patent Index (WPI), Accession No. 92-317846/39 (EP 504938), 1992.
Ando et al., WPI Accession No. 93-037845/05 (EP 525420), 1993.
Ando et al., WPI Accession No. 93-001259/01 (EP520427), 1992.
Keppler et al., WPI Accession No. 91-058150/08 (WO 91/01378), 1991.
Smith et al., J. Biol. Chem. 264:20487-20495 (1989).

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