Homologous recombination for universal donor cells and chimeric

Details Homologous recombination for universal donor cells and chimeric Homologous recombination for universal donor cells and chimeric

1993-12-30

1996-11-12

Chambers, Jasemine C.

Multicellular living organisms and unmodified parts thereof and

Nonhuman animal

424 92, 424 9321, 4351723, 4353201, 4352402, 800DIG1, 800DIG2, 935 62, 935111, C12N 1500, C12N 500, A61K 4800, A61K 4900

Patent

active

055742050

ABSTRACT:
Homologous recombination is employed to inactivate genes, particularly genes associated with MHC antigens. Particularly, each of the .beta..sub.2- microglobulin gene and the IFN-.gamma.R gene is inactivated for reducing or eliminating the expression of functional MHC antigens. The resulting cells may be used as universal donor cells. In addition, embryonic stem cells may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts, which may be used as source of universal donor organs, or as models for drug and transplantation therapies. Methods for homologous recombination in non-transformed mammalian somatic cells are also described.

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