Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1997-03-21
1999-12-07
Achutamurthy, Ponnathapu
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
4352523, 435194, 536 232, C12N 120, C12N 912, C07H 2104
Patent
active
059981954
ABSTRACT:
A plasmid for expression of Moloney Murine Leukemia Virus-derived reverse transcriptase in E. coli cells deficient in the expression of indiginous RNAse activity, a method for purification of the recombinant enzyme, and a composition comprising a cloned and purified reverse transcriptase opimized for use in cDNA and nucleic acid amplification procedures.
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Hautala, et al., Increased expression of a euraryotic gene in Eschcrichia coli through stabalization of its messenger RNA. Proc. Natl. Acad. Sci. USA 76(11):5774-5778 (1979).
Hu, et al., Murine Leukemia virus pol gene products: analysis with antisera generated against reverse transcriptase and endonuclease fusion proteins expressed in Escherichia coli. J. Virol. 60(1):267-271 (1986).
Kotewicz, et al., Cloning and overexpression of Moloney murine Leukemia virus reverse transriptase in Escherichia coli. 35:249-258 (1985).
Hizi, A. and S. Hughes, Expression in Escherichia coli a Moloney murine Leukemia virus reverse transcriptase whose structure closely resembles the viral enzyme. Gene 66:319-323 (1988).
Telesnitsky, A. and S. Goff, RNase H domain mutations affect the interaction between Moloney murine leukemia virus recerse transcriptase and its primer template. Proc. Natl. Acad. Sci. USA 90:1276-1280 (1993).
Amann, E. et al., Gene, vol. 69, pp. 301-315, 1988.
Durwald, H. et al., J. Mol. Biol., vol. 34, pp. 331-346, 1968.
Kacian Daniel Louis
Putnam James Garfield
Riggs Michael Garth
Achutamurthy Ponnathapu
Fisher Carlos A.
Gen-Probe Incorporated
Gritzmacher Christine A.
Tung Peter P.
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