Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Reexamination Certificate
2005-12-20
2005-12-20
Prats, Francisco C. (Department: 1651)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
C435S183000, C435S184000, C435S233000, C435S007400
Reexamination Certificate
active
06977142
ABSTRACT:
Methods for performing an end-point assay of protein disulfide isomerase activity. The method may be based on the enzyme-catalyzed reduction of insulin in the presence of dithiothreitol; measuring the aggregation of reduced insulin chains at 650 nm; and using hydrogen peroxide as a stop reagent.
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Huang Shu-Gui
Oksenberg Donna
Urfer Roman
AGY Therapeutics, Inc.
Bozicevic Field & Francis LLP
Fernandez Susan E.
Prats Francisco C.
Sherwood Pamela J.
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