Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1981-03-26
1986-06-10
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4351722, 435948, 935100, C12N 500, C12N 1500, C12R 191
Patent
active
045943257
ABSTRACT:
Human lymphoblastoid cell line capable of acting as a fusion partner in the preparation of hybridomas is grown and selected under conditions whereby a cell line is obtained having greatly enhanced fusion efficiency over the parent cell line. The cell line is derived from UC 729 by growing in Iscove's modified serum-free medium with plating at relatively high densities, followed by cloning at limiting dilutions and selecting for high frequency fusion.
The subject cell line referred to as WI-L2-729 HF.sub.2 has the A.T.C.C. designation number CRL 8062, having been deposited on Apr. 2, 1981.
REFERENCES:
Levy et al., "Further Characteraction of the W1-L2 and W1-L1 Lymphoblastoid Lines", Journal of the National Cancer Institute, vol. 46 (1971), pp. 647-652.
Croce et al., "Production of Human Hybridomas Secreting Antibodies to Measles Virus" Nature, vol. 288 (Dec. 4, 1980), pp. 488-489.
Ham et al., "Media and Growth Requirements" Methods in Enzymology, vol. 58 (1979), pp. 77-79, 90-93.
Iscove et al., "Complete Replacement of Serum by Albumin, Transferrin, and Soybean Lipid in Cultures of Lipopolysaccharide-Reactive B Lymphocyete, Journal of Experimental Medicine 147:(1978), pp. 923-933.
Kohler et al., "Derivation of Specific Antibody-Producing Tissue Culture and Tumor Lines by Cell Fusion," European Journal of Immunology, vol. 6 (1976), pp. 511-519.
Litwin, "A Survey of Various Media and Growth Factors used in Cell Cultivation," Developments in Biological Standards, vol. 42 (1979), pp. 37-45.
Rowland Bertram I.
Tarcza John Edward
The Regents of the University of Calif.
Wiseman Thomas G.
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