Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1997-03-24
2000-09-19
Woodward, Michael P.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
43524026, 4353201, 530350, 4241891, 4242281, C12N 1509, C12N 1574, C12Q 170, A61K 3929
Patent
active
06121020&
ABSTRACT:
Novel Hepatitis C E1 and E2 truncated polypeptides and complexes comprising these polypeptides, are disclosed. The polypeptides are C-terminally truncated to remove all or a portion of their membrane spanning domains. Hence, the polypeptides are capable of secretion when expressed recombinantly.
REFERENCES:
Nishihara et al., "Secretion and purification . . . ," Gene 129:207-214 (1993).
Choo et al., "Isolation of a cDNA Clone Derived from a Blood-Borne Non-A, Non-B Viral Hepatitis Genome", Science (1989) 244:359-362.
Choo et al., "Genetic organization and diversity of the hepatitis C virus", Proc. Natl. Acad. Sci. USA (1991) 88:2451-2455.
Choo et al., "Vaccination of chimpanzees against infection by the hepatitis C virus", Proc. Natl. Acad. Sci. USA (1994) 91:1294-1298.
Gething and Sambrook, "Construction of influenza haemagglutinin genes that code for intracellular and secreted forms of the protein", Nature (1982) 300:598-603.
Grakoui et al., "Expression and Identification of Hepatitis C Virus Polyprotein Cleavage Products", J. Virol. (1993) 67:1385-1395.
Han et al., "Characterization of the terminal regions of hepatitis C viral RNA: Identification of conserved sequences in the 5' untranslated region and poly(A) tails at the 3' end", Proc. Natl. Acad. Sci. USA (1991) 88:1711-1715.
Houghton et al., "Molecular Biology of the Hepatitus C Viruses: Implications for Diagnosis, Development and Control of Viral Disease", Hepatology (1991) 14:381-388.
Lanford et al., "Analysis of Hepatitis C Virus Capsid, E1, and E2/NS1 Proteins Expressed in Insect Cells", Virology (1993) 197:225-235.
Ralston et al., "Characterization of Hepatitis C Virus Envelope Glycoprotein Complexes Expressed by Recombinant Vaccinia Viruses", J. Virol. (1993) 67:6753-6761.
Rose and Bergmann, "Expression from Cloned cDNA of Cell-Surface Secreted Forms of the Glycoprotein of Vesicular Stomatitis Virus in Eucaryotic Cells", Cell (1982) 30:753-762.
Simmonds et al., "Classification of hepatitis C virus into six major genotypes and a series of subtypes by phylogenetic analysis of the NS-5 region", J. Gen. Virol. (1993) 74:2391-2399.
Spaete et al., "Characterization of the Hepatitis C Virus E2/NS1 Gene Product Expressed in Mammalian Cells", Virology (1992) 188:819-830.
Sveda, et al., "Cell Surface Expression of the Influenza Virus Hemagglutinin Requires the Hydrophobic Carboxy-Terminal Sequences", Cell (1982) 30:649-656.
Tomei et al., "NS3 Is a Serine Protease Required for Processing of Hepatitis C Virus Polyprotein", J. Virol. (1993) 67:4017-4026.
Matsuura et al., "Processing of E1 and E2 Glycoproteins of Hepatitis C Virus Expressed in Mammalian and Insect Cells," Virology 205(1):141-150 (1994).
Mizushima et al., "Analysis of N-Terminal Processing of Hepatitis C Virus Nonstructural Protein 2," Journal of Virology 68(4):2731-2734 (1994).
Selby et al., "Expression, Identification and Subcellular Localization of the Proteins Encoded by the Hepatitis C Viral Genome," J. Gen. Virol. 74(6):1103-1113 (1993).
Selby et al., "Complex Processing and Protein: Protein Interactions in the E2:NS2 Region of HCV," Virology 204(1):114-122 (1994).
Houghton Michael
Selby Mark
Blackburn Robert P.
Chiron Corporation
Harbin Alisa A.
Robins Roberta L.
Woodward Michael P.
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