Heat-stable recA mutant protein and a nucleic acid...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

Reexamination Certificate

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C536S023100, C435S071100

Reexamination Certificate

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07902335

ABSTRACT:
A heat-stable RecA mutant protein is obtained by mutation involving either deletion or substitution of at least one amino acid in an amino acid sequence composing an acid region at C-terminal end of a wild type heat-stable RecA protein, and has an improved ability, compared to the wild type heat-stable RecA protein, for contributing to an increase in an amplification specificity of a template nucleic acid in a nucleic acid amplification reaction.

REFERENCES:
patent: 5510473 (1996-04-01), Camerini-Otero et al.
patent: 2004/0081963 (2004-04-01), Wang
patent: 2005/0136443 (2005-06-01), Shigemori
patent: 2005/0260631 (2005-11-01), Shigemori et al.
patent: 2007/0092896 (2007-04-01), Shigemori et al.
patent: 1 522 597 (2005-04-01), None
patent: WO 2004/037979 (2004-05-01), None
Wetmur et al., Cloning, sequencing, and expression of RecA proteins from three distantly related thermophilic eubacteria., J Biol Chem, 1994, vol. 269, pp. 25928-25935.
Shigemori et al., Multiplex PCR: use of heat-stableThermus thermophilusRecA protein to minimize non-specific PCR products, Nucleic Acid Research 2005, vol. 33(14), pp. 1-9.
Celia Perales, et al., “Enhancement of DNA, cDNA synthesis and fidelity at high temperatures by a dimeric single-stranded DNA-binding protein”, Nucleic Acids Research, vol. 31, No. 22, 2003, pp. 6473-6480.
Quin Chou, “Minimizing deletion mutagenesis artifact during Taq DNA polymerase PCR byE. coliSSB”, Nucleic Acids Research, vol. 20, No. 16, May 28, 1992, p. 4371.
Rapley R., “Enhancing PCR amplification and sequencing using DNA-binding proteins”, Mol Biotechnol, 2(3), Dec. 1994, p. 295-8.
“iProof High-Fidelity DNA Polymerase”, Bio-Rad Laboratories Inc., 3 pages. last viewed on Jan. 6, 2006.
Yasushi Shigemori, et al., “Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products”, Nucleic Acids Research, vol. 33, No. 14, e126, 2005, 9 pages.

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