Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-09-20
1996-11-05
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 536 231, 536 2431, 536 2433, C12Q 168, C12P 1934, C07H 2102, C07H 2104
Patent
active
055716725
ABSTRACT:
A method for determining the genotype of a gypsy moth is disclosed. This method begins with the steps of isolating genomic DNA from a candidate gypsy moth, exposing that DNA to an oligonucleotide primer selected from the group consisting of SEQ ID NOs:3, 4, 5, 6, 7 or the complement thereof and sequences sufficiently similar to SEQ ID NOs:4 and 5 or the complement thereof under conditions permitting amplification of the genomic DNA, and comparing the amplified DNA with amplified DNA produced by control samples. A genotype identity is assigned to the candidate moth according to which control sample has amplified fragments similar to those in the candidate sample. A method for obtaining DNA primers for determining the genotype of candidate moths is also disclosed. This method comprises the steps of obtaining the DNA sequence of fragments FS-1, FS-2, or FS-3 from both the Asian moth DNA and North American/European moth DNA and analyzing the sequences to obtain primers capable of priming an amplified product of different size in Asian and North American/European gypsy moth DNA samples.
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Garner Karen J.
Schreiber David E.
Slavicek James M.
Fado John D.
Rees Dianne
Silverstein M. Howard
Stockhausen Janet I.
The United States of America as represented by the Secretary of
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