Gram-positive expression control sequences

Details Gram-positive expression control sequences Gram-positive expression control sequences

1986-06-17

1989-09-19

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 70, 435 91, 435170, 4351723, 43525231, 43525233, 435320, 435832, 435849, 536 27, 935 6, 935 8, 935 29, 935 39, 935 44, 935 61, 935 73, 935 74, C12P 2100, C12N 1500, C12N 120, C07H 1512

Patent

active

048681113

ABSTRACT:
New gram-positive expression control DNA sequences useful in the expression of pro- or eukaryotic proteins in gram-positive organisms are provided having in the downstream direction of transcription a transcription initiation DNA sequence of gram-negative origin combined with a ribosome binding site-encoding DNA sequence, optionally a foreign gene and a transcription termination sequence. Also described are expression vectors containing these expression control DNA sequences and processes using same for the manufacture of pro- and eukaryotic polypeptides. In addition processes for the manufacture of such gram-positive expression control DNA sequences and such expression vectors are described.

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