Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...

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47 58, 47DIG1, 4351721, 4351723, 4353201, 435419, 536 232, 536 236, 536 237, 800205, 800250, 800255, 800DIG9, 800DIG14, 800DIG17, 800DIG24, 800DIG26, 800DIG27, 800DIG43, 800DIG44, 800DIG57, 800DIG58, 800DIG63, A01H 106, A01H 500, A01H 510, C12N 514, C12N 1529, C12N 1531, C12N 1552, C12N 1582

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058667750

ABSTRACT:
Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthases, DNA encoding glyphsate-tolerant EPSP synthases, plant genes encoding the glyphosate-tolerant enzymes, plant transformation vectors containing the genes, transformed plant cells and differentiated transformed plants containing the plant genes are disclosed. The glyphosate-tolerant EPSP synthases are prepared by substituting an alanine residue for a glycine residue in a first conserved sequence found between positions 80 and 120, and a threonine residue for an alanine residue in a second conserved sequence found between positions 170 and 210 in the mature wild type EPSP synthase.

REFERENCES:
patent: 4769061 (1988-09-01), Comai
patent: 4971908 (1990-11-01), Kirshore et al.
Fitzgibbon (Dec. 1988) Ph.D. thesis, University Microfilms International, 1989, pp. viii-ix, 18, 22-29, 32, 93, and 96-108.
Potrykus (Jun. 1990) Bio/Technology 8:535-542.
Fillatti et al (Jul. 1987) Bio/Technology 5:726-730.
Botterman et al (Aug. 1988) Trends in Genetics 4(8):219-222.
DasSarma et al (Jun. 6, 1986) Science 232:1242-1244.

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