Glycosylated LPXTGases and uses thereof

Details Glycosylated LPXTGases and uses thereof Glycosylated LPXTGases and uses thereof

2003-10-01

2009-10-20

Saucier, Sandra (Department: 1651)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

Reexamination Certificate

active

07604975

ABSTRACT:
An endopeptidase is described which shows specificity for cleavage of a LPXTG (SEQ ID NO: 1) motif found in the cell membranes of gram positive bacteria, having an apparent molecular weight of 14,000 daltons, having a pH optimum of about 7.5 to 10, being salt sensitive, being heavily glycosylated, rich in alanine, lacking aromatic amino acids, having a Kmof 0.26 mM and having a backbone comprised of about 30% unknown amino acids, and containing carbohydrates that are essential for its activity. Methods of use and pharmaceutical compositions of this inhibitor are described.

REFERENCES:
patent: 5968763 (1999-10-01), Fischetti et al.
Watanabe et al. “Properties and lytic action of the P2-2 enzyme capable or lysing cells ofMicrococcus radiodurans” Agri. Biol. Chem. (1981) 45(5): 1215-1221.
Hung Ton-That, et al,PNAS, 96, 12424-12429 (1999).
Mazmanian, et al.,Molecular Microbiology, 40, 1049-1057 (2001).

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