Ginkgo biloba levopimaradiene synthase

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S232000, C536S023200

Reexamination Certificate

active

11166996

ABSTRACT:
The present invention is directed to nucleic acid sequences ofGinkgo bilobaditerpene synthases, particularly of a levopimaradiene synthase. More specifically, the invention is directed to a cell of a unicellular organism, such asSaccharomyces cerevisiaeorEscherichia coli,comprising levopimaradiene synthase for the metabolically engineered in vivo biosynthesis of a diterpene and a ginkgolide.

REFERENCES:
patent: 4849410 (1989-07-01), Jacobs et al.
patent: 5151352 (1992-09-01), Nakano et al.
patent: 5189187 (1993-02-01), Nakano et al.
patent: 5241084 (1993-08-01), Teng
patent: 5322688 (1994-06-01), Schwabe
patent: 5389370 (1995-02-01), O'Reilly et al.
patent: 5399348 (1995-03-01), Schwabe
patent: 5429939 (1995-07-01), Misawa et al.
patent: 5473057 (1995-12-01), Fenical et al.
patent: 5512286 (1996-04-01), Schwabe
patent: 5589581 (1996-12-01), Misawa et al.
patent: 5599950 (1997-02-01), Teng
patent: 5602184 (1997-02-01), Myers et al.
patent: 5637302 (1997-06-01), Bombardelli et al.
patent: 5637484 (1997-06-01), Yukimune et al.
patent: 5968789 (1999-10-01), Yukimune et al.
patent: 6235287 (2001-05-01), Weidner et al.
patent: 2003/0148479 (2003-08-01), Keasling et al.
patent: 2004/0005678 (2004-01-01), Keasling et al.
Albrecht, M., et al.,Synthesis of atypical cyclic and acyclic hydroxy carotenoids in Escherichia coli transformants, Journal of Biotechnology 58 (1997) 177-185, Sep. 22, 1997.
Bailey, James E.,Toward a Science of Metabolic Engineering, Science, New Series, vol. 252, Issue 5013, 1668-1675, Jun. 21, 1991.
Balz, Jean-Pierre et al.,Production of Ginkgolides and Bilobalide by Ginkgo biloba Plants and Tissue Cultures, Planta Medica 65, pp. 620-626, 1999.
Basson , Michael E., et al.,Identifying Mutations in Duplicated Functions in Saccharomyces cerevisiae: Recessive Mutations in HMG-CoA Reductase Genes, Genetics, 117, 645-655, Dec. 1987.
Basson, Michael E,Saccharomyces cerevisiaecontains two functional genes encoding 3-hydroxy-3-methylglutaryl-coenzyme A reductase, Proc. Natl. Acad. Sci. USA 83: 5563-57, 1986.
Bohlmann, Jorg et al.,Plant terpenoid synthases: Molecular biology and phylogenetic analysis, Proc. National Acad. Science USA, vol. 95, pp. 4126-4133, Apr. 1998.
Broun, Pierre et al.,Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids, Science Magazine 282, pp. 1315-1317, Nov. 13, 1998.
Cartayrade, Alain et al.,Ginkgolide and bilobalide biosynthesis in Ginkgo biloba.I: Sites of synthesis, translocation and accumulation of ginkgolides and bilobalide, Plant Physiol. Biochem. 13(11), pp. 859-868, 1997.
Corey et al.,Total Synthesis of (±)Ginkgolide B, J. Am. Chem. Soc. vol. 110, pp. 649-651, 1988.
Corey et al.,Total Synthesis of a C15 Ginkgolide, (±)—Bilobalide, J. Am. Chem. Soc. vol. 109, pp. 7534-7536, 1987.
Corey et al.,Total Synthesis of Ginkgolide A, Tetrahedron Letters, vol. 29, pp. 3205-3206, 1988.
Corey, E.J., et al.,Isolation of an Arabidopsis thaliana gene encoding cycloartenol synthase by functional expression in a yest mutant lacking lanosterol synthase by the use of a chromatographic screen, Proc. Natl. Acad. Sci USA vol. 90, pp. 11628-11632, Dec. 1993.
Crowley, James H., et al.,A Mutation in a Purported Regulatory Gene Affects Control of Sterol Uptake in Saccharomyces cerevisiae, Journal of Bacteriology, vol. 180, No. 16, pp. 4177-4183, Aug. 1998.
Funk, Christoph, et al.,Diterpenoid Resin Acis Biosynthesis in Conifers: Characterization of Two Cytochrome P450-Dependent Monooxygenases and an Aldehyde Dehydrogenase Involved in Abietic Acid Biosynthesis, Archives of Biochemistry and Biophysics, vol. 308, No. 1, pp. 258-266, Jan. 1994.
Hara, Mitsunobu, et al.,Leinamycin, A New Antitumor Antibiotic From Streptomyces, Producing Organism, Fermentation and Isolation, The Journal of Antibiotics, pp. 1768-1774, Dec. 1989.
Hezari, Mehri, et al.,Purification and Characterization of Taxa-4(5), 11(12)-diene Synthase from Pacific Yew(Taxus brevifolia)that Catalyzes the First Committed Step of Taxol Biosynthesis, Archives of Biochemistry and Biophisics, vol. 322, No. 2, pp. 437-444, Oct. 1, 1995.
Jiang, Yu, et al.,BTS1 Encodes a Geranylgerany1 Diphosphate Synthase in Saccharomyces cerevisiae, The Journal of Biological Chemistry, vol. 270, No. 37, pp. 21793-21799, Sep. 15, 1995.
Kajiwara, Susumu, et al.,Expression of an exogenous isopentenyl diphosphate isomerase gene enhances isoprenoid biosynthesis in Escherichia coli, Biochem J., 324(Pt 2): 421-6, Jun. 1, 1997.
Keasling, Jay, ““Degradation of Organophosphate Contaminants Synthesis of Isoprenoids,” Metabolioc Engineering of Microorganisms”, Nov. 11, 2000.
Keasling, Jay, “Metabolic engineering ofEscherichia colifor terpene production.” Metabolic Engineering, Feb. 18, 2004.
Kholodenko, Boris N., et al.,Metabolic Design: How to Engineer a Living Cell to Desired Metabolite Concentrations and Fluxes, Biotechnol Bioeng, 59(2):239-247, Jul. 20, 1998.
LaFever, Roy E., et al.,Diterpenoid Resin Acis Biosynthesis in Conifers: Enzymatic Cyclization of Geranylgeranyl Pyrophosphate to Abietadiene, the Precursor of Abietic Acid, Archives of Biochemistry and Biophysics, vol. 313, No. 1, pp. 139-149, 1994, Aug. 15, 1994.
Le Bars, Pierre L. et al.,A Placebo-Controlled, Double-blind, Randomized Trial of an Extract of Ginkgo Biloba for Dementia, J. Amer. Med. Assoc., vol. 278, No. 16, pp. 1327-1332, 1997.
Leak, Frank W., et al.,In Yeast, upc2-1 Confers a Decrease in Tolerance to LiC1 and NaC1, Which Can Be Suppressed by the P-Type ATPase Encoded by ENA2, DNA and Cell Biology, vol. 18, No. 2, 1999 pp. 133-139, 1999.
Learned, R. Marc, et al.,3-Hydroxy-3-methylglutaryl-coenzyme A reductase from Arabidopsis thaliana is structurally distinct from the yeast and anmal enzymes, Proc. Natl. Acad. Sci. USA vol. 86, pp. 2779-2783, Apr. 1989.
Lewis, T.L., et al.,Pleiotropic Mutations in Saccharomyces cerevisiae Affecting Sterol Uptake and Metabolism, Yeast 4(2):93-106, 1988.
Liu, Shuang-Jiang, et al.,A Novel Genetically Engineered Pathway for Synthesis of Poly(Hydroxyalkanoic Acids)in Escherichia Coli, Applied and Environmental Microbiology, vol. 66. No. 2, p. 739-743, Feb. 2000.
Martin, Vincent, et al., “Engineering a mevalonate pathway in Escherichia coli for production of terpenoids,” Nature Biotechnology, vol. 21, No. 7, pp. 796-802, Jul. 2003.
Misawa, Norihiko, et al.,Expression of a Tomato cDNA Coding for Phytoene Synthase in Escherichia coli, Phytoene Formation In Vivo and In Vitro, and Functional Analysis of the Various Truncated Gene Products, J. Biochem, 116, 980-985 (1994).
Misawa, Norihiko, et al.,Metabolic engineering for the production of carotenoids in non-carotenogenci bacteria and yeasts, Journal of Biotechnology 59 (1998) 169-181.
Misawa, Norihiko, et al.,Production of B-Caroltene in Zymomonas mobilis and Agrobacterium tumefaciens by Introduction of the Biosynthesis Genes from Erwinia uredovora, Applied and Environmental Microbiology, vol. 57, No. 6, p. 1847-1849, Jun. 1991.
Miura, Yutaka, et al.,Production of Lycopene by the Food Yeast, Candida utilis That Does Not Naturally Synthesize Cartenoid, Biotechnol Bioeng., 58(2-3): 306-8, Apr. 20, 1998.
Miura, Yutaka, et al.,Production of the Carotenoids Lycopene, B-Caroltene, and Astazanthin in the Food Yeast Candida utilis, Applied and Environmental Microbiology, vol. 64, No. 4, p. 1226-1229, Apr. 1998.
Neau, Elisabeth et al.,Ginkgolide and bilobalide biosynthesis in Ginkgo biloba. II: Identification of a possible intermediate compound by using inhibitors of cytochrome P-450-dependent oxygenases, Plant Physiol. Biochem. 35(11), pp. 869-879, 1997.
Ness, Frederique, et al.,SUT1 is a putative Zn[II]2Cys6-transcription factor whose upregulation enhances both sterol uptake and synthesis in aerobically growing Saccharomyces cerevisiae cells, Eur. J. Biochem. 268, 1585-1595, Feb. 2001.
Parks, Leo W., et al.,Biochemical and Physiological Effects of Sterol Alternations in Yeast-A Review, Lipids vol. 30 No. 3:227-230, 1995.
Parks, Leo W., et al.,Physiological Implications of Ster

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Ginkgo biloba levopimaradiene synthase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Ginkgo biloba levopimaradiene synthase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Ginkgo biloba levopimaradiene synthase will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3947137

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.