Gene transfer method

Details Gene transfer method Gene transfer method

2006-02-07

2006-02-07

Priebe, Scott D. (Department: 1632)

Chemistry: molecular biology and microbiology

Vector, per se

C435S325000, C435S455000, C435S456000, C435S457000, C435S462000, C435S466000, C424S093200

Reexamination Certificate

active

06995010

ABSTRACT:
A method of transferring a foreign gene into cells, characterized by involving: the step of transferring into the cells with the use of an adenovirus vector, a first nucleic acid, which has a sequence provided with adeno-associated virus-origin ITRs in both sides of the target foreign gene to be transferred, and a second nucleic acid, which has an adeno-associated virus-origin rep gene and a promoter for expressing this gene and carries a stuffer sequence inserted thereinto sandwiched in two recombinase recognition sequences and located between the rep gene and the promoter; and the step of expressing the Rep protein under the action of recombinase in the cells obtained in the above step to thereby integrate the target foreign gene into the chromosomal DNA.

REFERENCES:
patent: WO 9810086 (1998-03-01), None
Orkin et al., Report and recommendations of the Panel to Assess the NIH Investment in Research on Gene Therapy, issued by the U.S. National Institutes of Health on Dec. 7, 1995.
Verma et al., “Gene therapy—promises, problems and prospects,” Nature 389: 239-242, Sep. 18, 1997.
Rosenberg et al., “Gene therapist, heal thyself,” Science 287: 1751, Mar. 10, 2000.
Chejanovsky et al., “Mutagenesis of an AUG codon in the adeno-associated virus rep gene: effects of viral DNA replication,” Virol. 173 : 120-128, 1989.
Recchia., et al. “Site-specific Integration mediation by a hybrid adenovirus/adeno-associated virus vector,” Proc. Natl. Acad. Sci. USA 96:2615-2620 (1999).
Ogasawara, et al. “Highly regulated expression of adeno-associated virus large Rep proteins in stable 293 cell lines using the Cre/loxP switching system,” Journal of General Virology, 80:2477-2480 (1999).
Ueno, et al., “Site-Specific Integration of a Transgene Mediated by a Hybrid Adenovirus/Adeno-Associated Virus Vector Using the Cre/loxP-Expression-Switching System,” Biochemical and Biophysical Research Communications 273:473-478 (2000).

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