Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1995-06-07
1998-08-25
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
435190, 435191, 435193, 435232, 528361, C12P 762
Patent
active
057982350
ABSTRACT:
The present invention is a method for controlling biopolymer synthesis by determining the genetics and enzymology of polyhydroxybutyrate (PHB) biosynthesis at the molecular level. The purified enzymes and genes provide the means for developing new PHB-like biopolymers having polyester backbones. Specific aims are to 1) control the chain length of the polymers produced in fermentation processes through genetic manipulation, 2) incorporate different monomers into the polymers to produce copolymers with different physical properties, and 3) examine the physical/rheological properties of these new biopolymers in order to develop further design criteria at the molecular level.
The method for engineering biopolymer synthesis includes: isolation and characterization of the genes for the enzymes in the synthetic pathway (beta-ketothiolase, acetoacetyl-CoA reductase and PHB synthetase); cloning of the genes in a vector(s); placement of the vector(s) under the control of regulated promoters; expression of the genes; determination of the function and use of other factors such as substrate specificity in polymer production and composition; and isolation and physical and chemical analysis of the resulting polymers.
REFERENCES:
patent: 4477654 (1984-10-01), Holmes et al.
Peoples, O. P., et. al. (1987) J. Biol. Chem. 262 (1), 97-102.
Saito, T., et. al. (1977) Arch. Microbiol. 114, 211-217.
Fukui, T., et. al. (1987) Biochem. Biophys. Acta (1987) 917, 365-371.
Senior, P. J., et. al. (1973) 134,225-238.
Peoples Oliver P.
Sinskey Anthony J.
Massachusetts Institute of Technology
Patterson Jr. Charles L.
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